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...mentors and mentees have fulfilling, productive relationships. I will be sharing what I’ve learned along the...many had examples of very productive mentoring relationships with advisors and others who helped shape their...

...function, and C. elegans overexpression plasmids to upregulate their expression in specific cells. With a more...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...

...Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists ...resources on the Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19...

...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

...gives a presentation on what they do and how it relates to other teams at the company. This provides new... I know some fear that remote work would feel isolating, but that has not been the case for me at Addgene...

...citing plasmids from Addgene help others find them later if they need to use them in their own work, it also...should make it easier for them to find your work later. We also hope that this feature will decrease redundancy...

...distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously established...

...is pictured here…she started from a blank white slate and developed this over 30 minutes, which was fun...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...

...game for anyone looking to quickly and easily manipulate specific genes. But what if you want to study...said they were able to produce a very diverse population of cells within a lab dish in a matter of a few...

...Improvements In a world where funding cycles shift, regulations change, and supply chains strain, Addgene remains...breakthroughs. None of this progress happens in isolation. It’s made possible by a shared commitment to ...

...be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we ...size of the plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of...

...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...

... part of the job, but Caitlin doesn’t work in isolation. “A lot of what we do is work with students who...theses, and occasionally posting something science-related on Facebook. “I think this helped me say with confidence...

...identified proteins and enzymes. These compounds will later get tested for their potential agricultural and ...agriculture. For this project the expression of isolated bacterial proteins can be achieved using a bacterial...

...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

... left four years later with a master’s. At the time my degree felt like a consolation prize, but in time...

...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...

... data, and I was given a fantastic spreadsheet template to build out a report on. I was able to understand...cultivates trust between coworkers and that trust then translated into this incredible experience.  Of course, ...