We narrowed to 659 results for: MAL

1. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...light is in an open conformation.  Nanobodies are a small (~15 kDa), single monomeric variable heavy chain...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ... genes for noninvasive imaging of microbes in mammalian hosts Article contributed by Lukas Morgan  Listen...

3. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...to my mentors, as they encouraged me to explore small-scale opportunities where I could make mistakes ...

4. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...still continue gating populations of interest with normal gates, just based on a histogram plot instead of...

5. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   Published

   Feb. 19, 2019, 1:36 p.m.

   ...Businesswomen’s Association. We were involved in smaller group activities through the HBA and I got to know...

6. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ...delivering genes to many different cell types with minimal immunological effects. AAVs are used in experiments...

7. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   ... also false negatives: These are rare, but if a small fragment is inserted in-frame, read-through can ...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ... could typically achieve 90-99% knockdown with minimal off-target effects. The system also enabled them...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   Published

   Dec. 10, 2013, 3:13 p.m.

   ...one can introduce changes into the genomes of mammalian or other cells. TALENs are engineered proteins...

10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...should enable researchers to make precise edits in mammalian cells and may decrease worries about off target...

11. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...quantified for individual cells, and the number of 'normal' looking cells can be counted. This assay will ...

12. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ... faster presentation with more information than normal. I had to learn to observe and adjust on the fly...

13. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...better resolution as these immune complexes are smaller than a primary:secondaries:conjugates complex. ...

14. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...

15. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...pooled library of mutant sensors expressed in a mammalian cell line engineered to be electrically excitable...

16. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...further away. While constitutive promoters drove maximal gene expression near genomic origins of replication...

17. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...to use or build on work done previously, borrow small aliquots of restriction enzymes for one-off projects...

18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...great gene therapy delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery...

19. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...” “material,” “item,” and “format” all received formal definitions in our lexicon. We created documents...

20. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ...expression of ion channels, despite having an otherwise normal genomic background, can even lead to the formation...