We narrowed to 629 results for: Ncl

1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...historical studies of pathogen research have conclusively shown that barrier protocols are vulnerable ...

2. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use...

3. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   Published

   May 20, 2014, 2:06 p.m.

   ...encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 ...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ... molecules into regulation of gene expression including growth or fluorescence. There are a few examples...

5. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...confers resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic...

6. Getting to Know Expansion Microscopy

   Type

   Blog Post

   Published

   June 20, 2023, 1:15 p.m.

   ...insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biology 19:...

7. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ... ability to edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With...

8. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...transformation method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium...

9. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...identification of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive...

10. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...mammalian cells. The modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...various gram-negative bacterial hosts of interest, including within mixed communities and into species not ...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...PPIs that regulate numerous cellular functions, including ubiquitinoylation, kinase signaling, integrin ...

13. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ... multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...

14. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require a single-component...

15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...assigning a physical location to your samples, include as much detail about that location as possible....

16. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type

    Blog Post

    Published

    July 29, 2025, 1:15 p.m.

    ...PEG-mediated transformation into plant cells. Conclusion & Future Directions TnpB represents a new generation...

17. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...systemic changes, like using alt-text, captions, and inclusive design in our digital content. We have implemented...

18. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ...process. When enough copies of ATP-DnaA are bound, including at the low-affinity sites, the proteins work cooperatively...

19. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...host ranges. Popular replicons from these groups include: RK2 (IncP), RSF1010 (IncQ), and pSa (IncW)  (Schmidhauser...

20. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...about the whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage...