We narrowed to 632 results for: Ncl

...virtual and hybrid options is more accessible, inclusive, and accommodating for everyone involved in the...

...our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory...

...available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid...

...historical studies of pathogen research have conclusively shown that barrier protocols are vulnerable ...

...this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use...

...encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 ...

... molecules into regulation of gene expression including growth or fluorescence. There are a few examples...

...transformation method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium...

...identification of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive...

...various gram-negative bacterial hosts of interest, including within mixed communities and into species not ...

...PPIs that regulate numerous cellular functions, including ubiquitinoylation, kinase signaling, integrin ...

... ability to edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With...

... multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...

...confers resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic...

...insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biology 19:...

...mammalian cells. The modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation...

...Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require a single-component...

...assigning a physical location to your samples, include as much detail about that location as possible....

...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...

...can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal models, tissue...