We narrowed to 860 results for: PLE

... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...lab compared the efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

...looking to delve deeper or stay tuned, there are plenty of online resources available. I recommend a few...Science Translational Medicine have made their complete collection of relevant papers freely available...you have information or another resource to add, please tell us about it in the comments. ...

... Aavery in this Zoom video background. Want the purple bacteria video background with mascots Blugene ...and more!) on Addgene’s Help Center. Download the purple bacteria background.       Are none of those ...Addgene’s Science Careers blog topic. Download the purple lab-themed background.     We hope these science...

...template covalently attached to the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases...Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to high target...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...results Above (top right corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight...less and you might suspect contamination in your sample or consider asking your sequencing facility to ...Use a silica spin column for purification of the samples you send for DNA sequencing If your sequencing ...

... need a high efficiency of transformation (for example, if you are working with a library or an especially...making competent cells are available online. A few examples are provided here for reference: Untergasser ...transformation. This protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue,...

...scientific community? Access to plasmids which gives people more time to focus on their research.  How will...plan for moving forward. Sooner or later enough people will be vaccinated and we’ll have some herd immunity...raises questions like “How do you start to bring people back to on-site work?” and “How do you start to...

... of the coast of Washington state. Using these samples, he was able to isolate two proteins from the jellyfish...potential as a research tool. Soon, the number of people studying it grew. In particular, studies in the...organisms to find the culprits. He even obtained samples of certain ones from local pet shop aquariums in...

...Stefan Stricker’s lab, is a single step gRNA multiplexing system that generates vectors containing high...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...tested GreenGate in Arabidopsis, it should in principle work in any plant species. Tricks of the trade...essential for scientific progress. Also, the more people use such a system, the more building blocks will...

...interviewing fellow Addgenies. I spoke with amazing people that were a part of the different departments of...felt like I could actually make connections with people. These were some of the things that really made...by Addgene. I have met some of the most genuine people who have shared amazing advice with me. Due to ...

...team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate...bird feces, which get eaten by another snail, completing its lifecycle. Halloween genes The Halloween ...and drains all the blood out. Once the blood is depleted, the tongue atrophies and falls off. The louse...

...Science Communication group started. It's as simple as getting people together at a bar. Chapter 10: Maintaining...well as a platform for scientists to be viewed as people (#ActualLivingScientists). We also discuss the ...

...should receive the clinical sample to test their phages. Because the sample is limited, only the labs that...targeting organisms closely related to the clinical sample. For treatment, it is best to identify a cocktail...

...affected by devastating diseases that affect far fewer people and therefore fail to garner much international...the Institute of Biosciences and Bioresources in Naples, Italy. His group works on spinal muscular atrophy...data from primary peritoneal mesothelioma tumor samples that they have used to identify candidate genes...

...website as a “Publication Citing this Plasmid” - this simple act directly associates you with a network of researchers...functionality can be found in the following video: Please remember that the accuracy and reliability of our... plasmid is missing from our collections pages, please shoot us an e-mail at [email protected]. We’d love...

...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...quickly. Researchers should keep in mind that a very simple sequential or double restriction enzyme digest ...on the corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your...

...observe what’s happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those...

...helps improve your interpersonal relationships with people you’re collaborating with remotely. Depending on...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss...

...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...