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We narrowed to 790 results for: Por

Showing: 581 - 600 of 790 results

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...plotting the entire data set, plot only the linear portion. This allows you to use a simple linear regression... enough if your unknown lies within the linear portion of the graph. It does restrict the dynamic range...

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...channels both digitally and physically. Easy to incorporate visuals if needed. Traditional media formats ...assuming a high resource geographic location) Opportunity for real time engagement while allowing for recording...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration...al. Before picking a secondary nanobody, it’s important to review a few characteristics of your primary...

  5. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...

  6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...genomes engineering In the Sternberg group’s initial report of VchINT, three separate plasmids and multiple...simple to use, users should keep aware of a few important details. For transposons to be specifically recognized...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  9. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ... days to weeks in transit and some have been transported across oceans and continents. Each deposit may...misread.  2. Track the container - It is just as important to track the location of the container housing...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... are available, but scientists would need to incorporate their recognition sites into their protein tag...

  11. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a chimeric 5'LTR removes...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The deletion...

  12. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... perfect Our first tip, and arguably the most important, is to take some time to sit down, plan your cloning... plates of frozen glycerol stocks. It is very important that these stay frozen at -80 °C, since multiple...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...encountering flow data in the literature. Data reported from flow cytometry experiments can be a little...correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients. World...

  14. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...all of which need to be represented in the same proportion after amplification as they were prior to amplification...this guide, we hope you feel more confident incorporating them into your research. And, of course, if ...

  16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...sharing their ideas? And how do we give people the opportunity to speak out and prevent groupthink? Here are...implemented, impacted the entire company. It is important to note that the person who submitted the idea...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... More recently, the Bird lab found that only a portion of the MECP2 protein is necessary to rescue the...Neurological Disorder by Programmable RNA Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878...

  19. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...coding sequence. If you choose this strategy, it’s important to include the proper protein expression elements...Clone When making the expression clone, it is important to choose the destination vector that best fits...
Showing: 581 - 600 of 790 results