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We narrowed to 855 results for: REN

Showing: 581 - 600 of 855 results

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused” in ...self-police” or whether the media and academic conference response to He created a positive feedback loop...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ... improve tuberculosis care and control. In her current research, Sheen aims to identify new genes related...related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic...microfabrication technologies to generate a self renewable sensor, a bacterium that can sense contaminants...

  4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...academic community by funding their travel to a conference of interest. In this post, we’re proud to announce...for human milk oligosaccharides Fatima Enam is currently a graduate student in Thomas J. Mansell’s lab ...cost-effective way to measure the production of HMOs. Current HPLC-MS and fluorophore-based technologies are ...

  5. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...

  6. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...new and useful information, and invigoration - a renewed energy for continuing the project after leaving...a sense of the employee’s workload, updates on current projects, and offer feedback. The employee should...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...

  7. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...-friendly. They experimented with the promoter strength of various components, removing inducible promoters...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...introducing mutations (red nucleotide). Created with BioRender.com. The initial version of EvolvR has an editing...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

  10. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...

  11. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...

  12. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ... to its involved in cell proliferation and differentiation.  Since Ras is a membrane localized GTPase,...expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS activated...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...

  15. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...Association for Creatine Deficiencies (ACD) is a parent-let non-profit founded with the mission to support...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular...

  16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...data to prove it is worthless. The OSER assay is currently the best way to test a fluorescent protein’s propensity...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

  17. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...Rounding out the remainder, 2% of postdocs who weren’t going to get a salary increase prior to the injunction...that institutions have sent, with the lack of transparency and poor communication of their plans (many ...guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. ...

  18. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...has lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address...proteins that interact with an RNA of interest. Current labeling methods are not ideal for in vivo work...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

  19. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...sources. Mode of communication Different modes of communication take up different amounts of mental bandwidth... you're asking of your audience and when things aren't working out, adjust the ask.  I used to do a lot...

  20. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...recent positions outside of the lab. Granted, I currently work on the Scientific Support Team here at Addgene...resolves the issue.  If this sounds like about seven different skills to you, you are not wrong. Problem-solving...how you approach new challenges.     Know your strengths! Research experience presents ample opportunities...
Showing: 581 - 600 of 855 results