...control. A good positive control is bacteria transformed with the same backbone plasmid. If this control...strain. A good negative control strain is an untransformed culture of the same strain of bacteria you used...Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive Clones with Us ...

...responsibilities. Another tip: don’t miss your grandfather’s 100th birthday, as your mom will never forgive...when you can’t get home, babysitting or running errands. Give help when you have time, and in return you...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

...result from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be ...file. On the plot, the green (edited sample) and orange (control sample) lines should be close together...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...

...paid on fellowships instead of grants such that PIs no longer save grant money by paying relatively low...the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan...

...using the actual tool that you ordered and not a random aliquot pulled from a freezer. As you’ll see in...review our experimental designs and we need to be transparent about the reasons we alter these designs if we... wherein researchers can submit designs and transparently reveal them upon completing an experiment. There...

... to use CRISPR plasmids in the lab and the vast range of possibilities at their disposal. With this update...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe...

...facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ...

...Subscribe to the blog to stay in the know!     No pranks, just fun and games this April Fool’s Day on the... to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each photo, the pet...

...competitive field. Chapter 2: Wei Leong Chew's swift transition from graduate work to an independent position... Coming up with new project ideas, applying for grants, and mentoring. Chapter 5: Wei Leong Chew's lab...

...Troubleshooting Viral Transductions Getting the Most from Your Lentiviral Transduction Additional Resources...

...the interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History Rap Battle...telling following the unfortunate story of Rosalind Franklin and her incredibly important role in solving the...

...100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown announced...substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will allow us to...

...field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook....other Cas proteins continue to expand the targeting range. CRISPR can be difficult to deliver. Not all cells...

...diverse needs of our community and support a wide range of experimental designs. Here are nine great additions...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...

...Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4 - Developing...ensuring that success Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global...

...Sikora’s lab Matthew Sikora’s lab went all out to transform their lab into a walking tour of Buddy the Elf... care package from Addgene. We hope all of our entrants had fun decorating with their labmates! ...

...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...

...scientific breakthroughs, reagents, and know-how to transcend national borders and help people around the world...accelerate biology research in the labs they’re granted to, as well as shine a light on some of the great...

...light chains and two heavy chains. The ends of the branches contain the antigen binding sites and are highly...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...

...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...