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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 809 results for: TES

Showing: 581 - 600 of 809 results

  1. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...Pomodoro technique, which is when you spend 25 minutes focusing on a task, like writing, and then take...managers, but Mendeley is free to use. You can take notes and highlight the papers saved to your Mendeley ...

  3. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...

  5. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...site of interest, which nCas9 nicks and then dissociates from. PolI3M then binds the nicked DNA, and extends..., where it nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the ...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

  11. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides... and fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering...

  12. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen Elledge. His new ...fragments without the need for specific restriction sites or DNA ligase! This post was originally written...

  13. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

  14. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...security-critical bugs, and contribute to constant updates of the software. In hardware, this process is often...are as follows: Detailed documentation, which facilitates the reproduction of the structure in a similar...

  15. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...small molecule realm is typically not feasible to test in any given screening experiment. Thus, a limited...require there to be a culture condition which eliminates manipulations that do not affect the function...

  16. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...parts: the receptor itself and the G-proteins it associates with.  Receptor: GPCRs are also known as seven...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...

  17. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...behind Addgene’s new search  Like millions of other websites, we built our new search engine atop Elasticsearch...

  20. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...
Showing: 581 - 600 of 809 results