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We narrowed to 867 results for: TOR

Showing: 581 - 600 of 867 results

  1. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...help win a business plan competition and advance investors to the next stage of diligence." In business competitions...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing things...crowd funding, federal funding, foundations, and investors are examples of various sources of funding. Always... Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack ...

  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...Engineering gut microbes in situ While the ability to monitor the microbiome with sequencing is vast, the ability...upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin with the...origin with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by... Counting cell division in the gut microbiota Monitoring microbial population dynamics in the gut can ...

  3. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors guide Find our chemogenetics...makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins...non-invasive methods to the neurons in the right secondary motor cortex (M2). Since M2 is located on the surface ...

  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...support team, is a safer bet than one from a large distributor that may not have direct experience with the ...decision by price point, shipping time, or other factors. Just remember that these are secondary considerations...specificity, and experimental considerations - no other factors can make up for an antibody that doesn't work for...that application validation is the most important factor in antibody selection, but what does that mean?...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...into mammalian cells, especially when fused to effector proteins for gene activation or gene editing. ... efficient Cas system with very compact genome editors and a gRNA down-sized by almost 40%. The activity...architecture and amino acid sequence of the prime editor, yielding PEmax, to work synergistically with epegRNAs... within the FAH gene was precisely corrected, restoring expression in the liver.  Read the paper in Nature...

  6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...New Under The Sun Blog and is part of her larger tutorial series, A Beginner’s Guide to Writing Scientific...when I can’t it’s an easy search. Writing this tutorial has shown me that I’m still in the dark ages with...guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences at Louisiana...Manuscript by Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

  7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ... infections along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential...plasmids from Addgene to construct NFIX expression vectors as well as reporters of NFIX function. They will...

  8. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...design was conceived by Mike Morrison, a psychology doctoral student at Michigan State University. The idea... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...be the focus of your poster - the data and your story. If you choose a single-color background but want...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ...depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi using four-dimensional contrast...assembly line for the creation of CRISPR targeting vectors that each contains a guide RNA and repair template...  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc- 119 +...

  10. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...suite, with Beam Therapeutics initiating a base editor-based clinical trial for SCD in 2022 and Editas... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  11. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...compared to the information provided by the depositor. If a depositor provides a sequence for the plasmid, QC...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains... elements based on the information provided and vector type  Any tags, selectable markers, and fusion ...

  12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...student and I truly enjoyed learning.” Addgene’s Director of Product Management Eric Perkins, who has been...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal...Komuhendo, a Lab Technician, dreamed of becoming a doctor. After taking a year break in between undergraduate...interviewees. Fabienne Furt, Addgene’s Associate Director of Business Development, got her PhD in Plant ...

  13. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ a previous supervisor / someone...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...

  14. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...to) feel valued. Talk to your manager and find a mentor Your manager should support you, just like you ...reaching out to your manager. This is when having a mentor - someone more experienced to guide you - is valuable...Management for Scientists Series What Makes a Good Mentor? Tips for Working in Industry and Success in Management...

  15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...subcloned (using FX cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag... and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound spike... Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101....16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L...

  16. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very predictable ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...research moving. Below, you’ll find some great introductory resources. As you dive deeper into Drosophila... the upper right hand corner. They have video tutorials under the help tab like this one to get you started...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...thankfully, most competent cells do not express this factor. Zeocin is handy because it can be a one-stop shop...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...bonded proteins in its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  19. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...team compares it to the plasmid information or depositor-provided sequences to confirm the correct plasmid...them. DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the... full-length predicted sequences submitted by depositors. A sample will automatically pass through our...(1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences...

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...bacteria, however, the protein integration host factor (IHF) assists with the process: it bends the DNA...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing (‘handcuffing’) ...
Showing: 581 - 600 of 867 results