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We narrowed to 856 results for: TOR

Showing: 581 - 600 of 856 results

  1. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...compared to the information provided by the depositor. If a depositor provides a sequence for the plasmid, QC...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains... elements based on the information provided and vector type  Any tags, selectable markers, and fusion ...

  2. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ... infections along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential...plasmids from Addgene to construct NFIX expression vectors as well as reporters of NFIX function. They will...

  3. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...suite, with Beam Therapeutics initiating a base editor-based clinical trial for SCD in 2022 and Editas... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ a previous supervisor / someone...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...subcloned (using FX cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag... and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound spike... Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101....16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L...

  6. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...to) feel valued. Talk to your manager and find a mentor Your manager should support you, just like you ...reaching out to your manager. This is when having a mentor - someone more experienced to guide you - is valuable...Management for Scientists Series What Makes a Good Mentor? Tips for Working in Industry and Success in Management...

  7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very predictable ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...research moving. Below, you’ll find some great introductory resources. As you dive deeper into Drosophila... the upper right hand corner. They have video tutorials under the help tab like this one to get you started...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...thankfully, most competent cells do not express this factor. Zeocin is handy because it can be a one-stop shop...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...bonded proteins in its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... weird black pellet's DNA yielded a new CRISPR effector protein they named Cas7-11. Testing showed that.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature. 2021 Sep;597(7878):720-725. doi...

  11. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...bacteria, however, the protein integration host factor (IHF) assists with the process: it bends the DNA...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing (‘handcuffing’) ...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...possess resistance genes survive and multiply. Factors contributing to the development of resistance include...www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely take time to determine if ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent... of fluorescent proteins derived from Aequorea victoria GFP sticks out and can be considered a linker ...Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric G-proteins...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

  14. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...due to loading and transfer errors — not to any factors from before the proteins even hit the gel. But ...all, while overloaded proteins may saturate the detector. In between these extremes lies the linear detection...concentration as well as by antibody dilution — both factors we’ve covered in our previous blog posts (1, 2)...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...site of interest can be cloned into an expression vector with Gibson assembly. While this is a comprehensive...is generated by transforming a backbone phagemid vector into an E. coli strain that is deficient in dUTPase... combine pairs of mutations together to test combinatory effects. One-pot saturation mutagenesis The ...2 instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...

  16. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-BioID combines... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins... in one experiment a scientist can confirm and monitor the interaction of two proteins while also biotin...Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein...

  17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...have previously used histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...TALENs focused primarily on disrupting CCR5, the receptor that HIV-1 uses to enter a cell. ZFNs and CRISPR...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

  18. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...reduced by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...ladder runs off the gel. Luckily, this is easily monitored in real time, as the dye front (and many ladders...resources with examples, like BioRad’s Western Blot Doctor page, or robust discussions, like ResearchGate’...longer time Protein degradation Add protease inhibitors to lysate Insufficient denaturing conditions...

  19. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...to sort cells based on the expression of a new receptor that is of interest to you. You found a couple...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...understand if protein:protein interactions could be a factor in your analysis. Example: You are planning an ...Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R, Turley H, Pulford K, Banham AH (2015) The European...

  20. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    Published
    Sept. 7, 2021, 1:15 p.m.
    ...protocols for plasmid cloning and working with viral vectors, but what we lacked were videos guiding completely...completely new members of the lab or those taking introductory lab courses. The Intro to the Lab Bench series... need a refresher on some best practices for introductory science techniques. It’s also great for teachers...
Showing: 581 - 600 of 856 results