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...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

...defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). The reversion of this...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...

...alongside standard surface labels. Here, I will introduce you to a few useful labeling and staining techniques...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

...If you read my eBook and it helps you, please introduce yourself to me by email or in person. If you feel...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the...

...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...women), visibility (how to get it, especially if introverted ), and networking (how to get people to remember...

...exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or absence of dNTPs. Secondly...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...

...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...

...Structure-guided directed evolution If you desire a bit more control, structure guided directed evolution might be more...evolution, all while maintaining a higher degree of control and efficiency! Screening mutant libraries After...

...culture spilled immediately. Water baths, liquid nitrogen containers, reagent bottles and other sources ...Mycoplasma Infections from Continuous Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290...

...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

...constructs were designed to be packaged into a retrovirus for infection of host cells. The fluorescent ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...

...Research requires dedication and effort, so building a strong understanding of what you’re getting yourself into... what I don’t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what...

...specify and correct single-base mutations without introducing DSBs at all. Base editing to the rescue! Base...editor. Dotted red arrows indicate the change introduced to the opposite strand following base editing...

...immunocytochemistry, and flow cytometry. It's a great introduction to — or quick refresher on — these common antibody...

...nomenclature to popular cloning techniques and troubleshooting tips. The fourth edition has been fully updated...

...some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this...

...scientists everywhere. In it, you can find an introduction to viral vectors and their elements; resources...

...resources on the Addgene blog Antibodies 101: Introduction to Antibodies New Antibody Animation ...