We narrowed to 642 results for: VARS

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...sometimes limited across different targets due to variations in chromatin context. Our recent studies (Mathis...

2. Antibodies 101: Flow Compensation

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   Blog Post

   Published

   May 16, 2024, 1:15 p.m.

   ... research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene...

3. Plasmids 101: Knockout/Knock-In Plasmids

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   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...depositors have developed new CRISPR-based methods for various knock-ins, discussed in our CRISPR 101 eBook. ...

4. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ... Various Addgenies...

5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ...expression. They found that the cellular fitness cost varied greatly between chromoproteins. In some cases, ...

6. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...training from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ ...

7. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   .... Despite the prevalence of Ras mutations in a variety of cancers, Ras proteins have stumped the pharmaceutical...

8. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...

9. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP....

10. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ..., and the balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a...

11. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    Published

    Feb. 4, 2016, 3:30 p.m.

    ...Check the Price of course! The price of some items vary, for example, plasmid kits are avaliable for less...

12. Genome-wide Screening Using CRISPR

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    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...modifying the genome. If you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR...

13. Light Sheet Fluorescence Microscopy

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    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ... reviews on several other LSFM configurations’ various features see (4,7).   Light sheet fluorescence ...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the protein-interaction...

15. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ...researchers have begun to make important discoveries in various biological fields. In 2015, the Fujii Lab identified...

16. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...different genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...

17. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    Published

    Jan. 16, 2020, 2:18 p.m.

    ...ddPCR. One issue with qPCR is that the results can vary by a factor of 2. This means that if you set up ...

18. Tips for arabidopsis transformation

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    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ... or affinity tags (HA, FLAG). There are also a variety of plant selection markers (basta, hygromycin, ...

19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with...

20. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ... based on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their ...