Hot Plasmids and Viral Preps - May 2021
Type
Blog Post
Published
May 18, 2021, 1:15 p.m.
... Various Addgenies...
We narrowed to 649 results for: VARS
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Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....nuclease-induced gene disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....expression. They found that the cellular fitness cost varied greatly between chromoproteins. In some cases, ... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....depositors have developed new CRISPR-based methods for various knock-ins, discussed in our CRISPR 101 eBook. ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m..... Despite the prevalence of Ras mutations in a variety of cancers, Ras proteins have stumped the pharmaceutical... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP.... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m...., and the balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....Check the Price of course! The price of some items vary, for example, plasmid kits are avaliable for less... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade plasmids ... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....that worked with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m.... this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....for easy screening of the insertion event, and a variety of connector modules (linker sequences between ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....training from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ ... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....MOI optimization to account for batch-to-batch variability. Check out this post to learn more about different... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....versatile and efficient way to generate a wide variety of constructs. Similar, Gateway cloning (discussed... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....sometimes limited across different targets due to variations in chromatin context. Our recent studies (Mathis... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m.... research technician in Arlene Sharpe's lab at Harvard Medical School. More resources on the Addgene... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....modifying the genome. If you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m.... reviews on several other LSFM configurations’ various features see (4,7). Light sheet fluorescence ...