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We narrowed to 807 results for: app

Showing: 581 - 600 of 807 results

  1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... for non-neural cells, ion-based communication happens at a slightly slower scale.  All living cells ...After attending this meeting I had an increased appreciation for the role of ion channels and pumps in developmental...

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this...

  4. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

  5. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail, diseases and...Fragile X syndrome, etc.). Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

  14. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...found that at least 50% of on-target Cas9 edits happens within the first six hours. When added to human...B, Bondy-Denomy J (2020) Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies....

  15. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer...

  16. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...

  17. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used to non-invasively...

  18. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ...

  20. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...
Showing: 581 - 600 of 807 results