We narrowed to 718 results for: cel
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TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...
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Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on... Sharing this story is a truly special way to celebrate our twentieth anniversary in 2024, and it’s all... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m.... attempt to write grants. If you don’t enjoy or excel at writing, an industry postdoc will free you of...Scientist at AstraZeneca. She has a background in cell and developmental biology and is applying this towards... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....analyzed using Addgene’s Analyze Sequence tool. Excellent, how can I check if the plasmid will be within...such as some of our viral vectors, in NEB Stable cells. Resource information: This section contains information... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....transparent are enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented... Weber, M. Mickoleit, J. Huisken. (2014) Methods Cell Biol.123, 193-215. Pubmed. W. Spalteholz. (1914)... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....incubated at stationary phase for too long, the cells in your culture will no longer be viable. The time...graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage and has... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m.... this, you can increase the volume of competent cells you plate after each transformation and/or use different...recommend transforming into chemically competent cells in a 96-well PCR plate. Always confirm your results... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....examples could be an endogenous sample of wild type cells, purified protein, or a peptide. The positive control... target antigen, such as a knockout or knockdown cell line or tissue samples where the target is not expressed... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....mission and our own, as both address the need to accelerate science using different approaches. We were also...evolved into a single, organized pipeline and accelerated the processing of our sequencing data. However... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....a review article on histone modifers in B and T cells with my adviser when I was a graduate student. Naturally...acetyltransferases and histone deacetylases in B-and T-cell development, physiology and malignancy." Genes &... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m.... the 926 video abstracts identified. The journal Cell had the largest average view count per video abstract.../wesharescience.com/pin/7472 Article: http://www.cell.com/current-biology/abstract/S0960-9822(16)30873... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....ability to establish and maintain itself within host cells. The plasmid has multiple ori that are functionally...eutrophus, Bartonella bacilliformis, Bordetella spp., Brucella spp., Caulobacter crescentus, Escherichia coli... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....particles that can transduce cells. Infectious titers are typically quantified by cell transduction assays. Wild-type... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....adopting simple note-taking tools, others using excel sheets or organizing folders in complex ways, there...store everything in one place. From pictures to excel sheets, researchers today generate vast amounts ... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m.... Zooming Forward Design: We produced a video celebrating Massachusetts Nonprofit Day, which was the perfect... Quintin Marcelino... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....is immediately ready to transform into competent cells. The master mix of enzymes can be purchased from...mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....ligase bound to nicked DNA-adenylate." Molecular cell26.2 (2007): 257-271. PubMed PMID: 17466627. 25. Luo...electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes... -
A Case Study of Addgene Bioinformatics
TypeBlog PostPublishedMay 25, 2023, 1:15 p.m....evolved into a single, organized pipeline and accelerated the processing of our sequencing data.” Recently... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog PostPublishedNov. 30, 2015, 3:30 p.m....by snapping a photo of you and your labmates celebrating the holidays and tweeting it with the tags #DeckTheLab...