Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 811 results for: cha

Showing: 581 - 600 of 811 results

  1. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...something that broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...fluorescent measurements from red, blue, and green channels overlaid on a bright-field image. Image from Ma...intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. CRISPRainbow: Scaling up to...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ..., and is long enough that there is virtually no chance of it occurring randomly. Therefore, inserting ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

  5. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...C.elegans neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle ...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...derived from random crosses between mice of uncharacterized backgrounds to create a limited but genetically...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...confluent and the media will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week...

  9. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...spirit include: Start using electronic multichannel and single channel pipettes. These can speed up any project...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...Building with Biology. Local environmental groups, charities and NGOs are often looking for outreach volunteers...Answering the big science questions around climate change, biodiversity, or genomes requires lots of data...

  11. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... wells with a purified protein allows you to characterize properties of an antibody that recognizes that.... As mentioned above, it can also be used to characterize antibodies against the antigen bound to the ...

  12. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...edited and the sequence of the edit, so its design changes for every new prime editing application. And optimizing... L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R....

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...nucleotides are represented equally, that’s only a 1/16 chance of having a PAM at a given site. While it is possible...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...was limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12...and screening that led to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco...

  15. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of the TTR protein, which contributes...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding and therefore..., P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques ...

  17. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...With the pseudovirus, scientists can study the mechanisms of infection and how to interfere with it in ....org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub JM, DiVenere AM, Kuo H-C, Javanmardi K, Le KC, ...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to ...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...semi-circle (so they can see each other) on comfortable chairs (stools if you have to, but this is tough for women...things started. Give a couple of audience members a chance to participate. For example, at a recent event ...

  20. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...attending on the larger side? At large conferences, chances are that several talks will be going on at once... for myself like meet ten new people each day. Exchange business cards. When you’re done networking for...
Showing: 581 - 600 of 811 results