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We narrowed to 818 results for: cha

Showing: 581 - 600 of 818 results
  1. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...MTA system. We also started our blog and YouTube channel in 2013, which continue to grow today. “I generally...Neuroscience 2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene...
  2. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking to modify...it perfect for targeting mRNA and useful as a mechanism of transient knockdown of gene expression. This...
  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...
  4. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...common method. These proteins bind the IgG heavy chain. Running the serum over a column of resin crosslinked...low abundance or whose conformational state may change. If your protein might become slightly denatured...
  5. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...something that broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...
  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ..., and is long enough that there is virtually no chance of it occurring randomly. Therefore, inserting ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...
  7. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...Building with Biology. Local environmental groups, charities and NGOs are often looking for outreach volunteers...Answering the big science questions around climate change, biodiversity, or genomes requires lots of data...
  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...confluent and the media will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week...
  9. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...spirit include: Start using electronic multichannel and single channel pipettes. These can speed up any project...
  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...C.elegans neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle ...
  11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...derived from random crosses between mice of uncharacterized backgrounds to create a limited but genetically...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...
  12. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...edited and the sequence of the edit, so its design changes for every new prime editing application. And optimizing... L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R....
  13. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding and therefore..., P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques ...
  14. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...nucleotides are represented equally, that’s only a 1/16 chance of having a PAM at a given site. While it is possible...
  15. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...was limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12...and screening that led to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco...
  16. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of the TTR protein, which contributes...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...
  17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... wells with a purified protein allows you to characterize properties of an antibody that recognizes that.... As mentioned above, it can also be used to characterize antibodies against the antigen bound to the ...
  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...With the pseudovirus, scientists can study the mechanisms of infection and how to interfere with it in ....org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub JM, DiVenere AM, Kuo H-C, Javanmardi K, Le KC, ...
Showing: 581 - 600 of 818 results