...demonstrate the most exciting application for ARG’s: in vivo imaging. Specifically, arg1 expressing E. coli... interpret signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging...intestinal tract and has been used for 100 years to clinically treat enteric infection and inflammatory bowel...abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to show where the bacteria...

...sequences To design experiments for further plasmid verification Identifying plasmid features While our quality...promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included...reference sequences. Using Snapgene for additional verification experiments: Primer and restriction digest design...repetitive regions. These regions require additional verification experiments that can be partially designed using... plasmid verification...

...be used to generate organoid cultures with a modification of interest. While the authors optimized the...mechanisms related to morphogenesis, cell fate specification, and human disease.     Sun, et al. bioRxiv...fluorescent biosensor based on the parasitic Striga hermonthica SL receptor HTL7. This novel type of biosensors...could be used in high throughput screening for agrochemical compounds that have the potential to regulate...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...

...protein transducers and that the potency and efficacy is typically non-uniform across the transducerome for...TRUPATH collection allows researchers to measure the physical association of combinations of different Gα, Gβ...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding...Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...

...re proud to honor the creativity, passion, and dedication to sharing epitomized by the creators of these...plasmids through our repository since we know biological resource centers, including Addgene, positively...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...Since we so sincerely appreciate our depositor’s dedication to scientific sharing, we wanted to celebrate.... “Addgene has made it possible to eliminate geographical restrictions in life science research." Claim...

...All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness...Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects. Other young...scientists discussed new CRISPR delivery systems, modifications to the RNA components, and challenging target...discussed some of the many challenges of creating biological circuits, and I can only say this science is ... deposited and distributed – have changed so dramatically in the past 4 years. I kept busy for the whole...

...while constant regions are identical for antibodies within certain classification groups called isotypes....published, knockout validated, and work in your application. But…they are the same isotype and therefore ...antibodies are plasmid-based and are created synthetically or derived from hybridoma antibody sequences...antibodies affects binding properties. In Journal of Biological Chemistry (Vol. 269, Issue 17, pp. 13048–13055...

...template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo ...differences in cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage...site specified by the gDNA. Depending on your application, this could be favorable or unfavorable. In a...PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-... PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute...

...analyzing the gene’s function. The process is shown graphically in the following figure, in which we add EcoRI...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...need to use the reverse complement to get PCR amplification. We can start similarly, taking the final 18...reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready for restriction...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

...antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-organized, documented... sample prep. This involves using heat and/or chemicals to break some of the bonds keeping the protein...epitope (right). Created withBioRender.com.   Biological Processes  If you’re feeling relieved that you...the available epitopes could be affected by, or critical to, the assay.   Next, look for antibodies that... also working for a Western because they both typically use denatured protein samples. If, however, you...

...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...resource of over 100 entries is driven by the dedicated community of data-contributing researchers who...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get... we’d like to give a shoutout to all of those dedicated scientists who have contributed to the AAV Data...

...culture), which is great for a wide variety of applications. But some techniques require you to select against...antibiotic. Successful editing of transformants then typically occurs in T0 plants (from callus) or T1 plants...cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments found...these mutant plants. Using RUBY to simplify the identification of these mutants will speed up my work and ...student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology Department...

... The conventional approach has typically been to either systematically delete/overexpress endogenous genes... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-...

...particle to infect a cell, it has to come into physical contact with that cell. Since there is a limited... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...AAV-mediated gene expression in a target cell. Practical considerations If you have high quality AAV, you...Integration. Molecular therapy : the journal of the American Society of Gene Therapy. 2008;16(7):1189-1199.... Protocols for Work with Virus Watch Our AAV Purification Video ...

... Writing schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for...app lets you track habits, like writing. It’s basically the equivalent of crossing off days on the calendar...suggestions Hemmngway makes (it wasn’t designed for technical writing), it’s great at flagging passive voice...jargon, this tool can help you avoid overusing technical terms when writing for a general audience. The...goal of the phrase, such as being cautious, being critical, or defining terms. The phrases in Phrasebank ...

...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...backgrounds.” This work outlines the need for clear communication, fair credit through authorship, and acceptance...remotely. The authors stress the fact that “miscommunication, a lack of leadership, and inappropriate tools...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...setting. Check out the blog post “Fostering Communication in Remote Collaborations” to get more ideas....

...imaging apoptosis in animals." Journal of the American Chemical Society (2019). PubMed PMID: 30821975. Additional...cell death" plays a pivotal role in an array of biological processes including development, the immune system...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters... reporter for imaging apoptosis in vivo." Cell chemical biology 23.7 (2016): 875-882. PubMed PMID: 27447051...

... Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the...environment for research is totally dependent on communication amongst the team so getting everyone talking...your department, and online. The Howard Hughes Medical Institute (HHMI) offers some great resources for...Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and...

... the steps of gel purification and in vitro recombination. Across all modifications described in Trehan...effects of inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM...mutations, large deletions/insertions require complicated, often costly in vitro assembly methods. A new...recombination machinery, which facilitates easy genetic modification by homologous recombination. In most recombineering...

...North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the Allen ...directions. I would like to thank them for their time, dedication, advice and encouragement. Why virus? Why did...educational materials, and provides viral vector technical service (details about virus production protocols...word out about this new resource and providing fantastically useful educational content on viral vectors....the impression that it took a lot of work and dedication to do this well ... because it did. So finally...