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We narrowed to 845 results for: ins;

Showing: 581 - 600 of 845 results

  1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...individual plasmids to 5,000 different research institutions. This has given us a unique window into technology... life sciences. In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed...journey since starting Addgene. Thank you for inspiring us with your breakthrough research and your commitment...

  2. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ... you already have a transgenic mouse line that contains a Cre-dependent mCherry cassette under the control...of these options would be very time consuming. Instead, a quick way to get the results you need would ...complicated ways to use this particular AAV. For instance, you might use it as part of a flex switch you...

  3. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... I was able to express my visions for what my Instagram posts would look like. While working remotely ...continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role... Some of recent Meet an Addgenie posts on Instagram! From this project, I observed what it was ...

  4. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...the page looked clean, professional, and modern. Instead of trying to place all of the content above the... regularly be paired with a variety of science-inspired friends. As you can see on the homepage, Blugene... you can find what you need and feel happy and inspired to tackle your next science challenge. If you ...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...plant species. Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium...promoter has been modified with duplicated enhancer domains and characterized in studies of tobacco, Arabidopsis...regions permit multiple regulatory elements to be inserted, further customizing gene expression. Sahoo et...

  6. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...audience to use their useful energy and to choose institutions with allies engaged in training excellence to...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs... training programs (like at the Keck Graduate Institute), funding for training (such as the NIH BEST award...

  7. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...and connections that emerge during moments of inspiration. Additionally, journaling in the morning has ..., whether within academia or industry. Albert Einstein once said: “There are only two ways to live your...

  8. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...systems. As a part of this effort, members of the Rawlins Lab have compiled a new toolkit for performing ...SL fluorescent biosensors by integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control...in tobacco protoplasts, the enzymatic biosensor retains its catalytic activity.  As SLs are also known ...

  9. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...calculators These links can help you calculate how much insert and backbone you need for different types of cloning...method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators...IHC.  FPbase: A data repository for fluorescent proteins and their properties.  Expasy: Bioinformatics ...

  10. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...out the Cellosaurus FAQ! References Lorsch JR, Collins FS, Lippincott-Schwartz J. Cell biology. Fixing...

  11. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...activation to behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The...by working with organisms with light accessible brains, such as zebrafish larvae. Mouse studies, however...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...

  12. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...favoring the separation of the two helixes. Low pH instead results in the breakage of the ester bonds responsible...the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification are endA negative...mutated to neutralize the nuclease activity. For strains that still carry this gene, there are usually extra...

  13. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...is expressed. In cells where the stop cassette remains, the effector is not expressed and the cells are...leads to gene constructs where the stop cassette remains following recombination. This strategy results ... Aliyah Weinstein...

  14. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...bacteria. Until recently the only available Cas9 proteins were isolated from mesophilic (medium heat loving... as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use...when incubated in diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human...

  16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...use two antibodies - one against your target of interest and a second against the first antibody. When.... (C) A third solution is to use Fab fragments against the sample species to block the binding sites on...

  17. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...this is a much-needed community resource. Instead of painstakingly searching for experimental details in ...serotype, promoter, or tool type and each entry contains an image along with key experimental details (...

  18. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this person ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ...interviewed for 1 hour so having a broad enough insight was going to help me push both my scientific and...

  19. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...was robust, capable of generating a wide range of insert sizes and also produced high yields of quality ... an NEB drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the...being overwhelmed by all the new information, and instead allow me to pay attention and answer my key questions...

  20. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...platform to help researchers working on COVID-19. Its origins trace back to March 2020, when the first coronavirus...use this potential to make a difference not just against COVID-19, but also in other areas of scientific...Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL...
Showing: 581 - 600 of 845 results