We narrowed to 829 results for: lars

...known to have replicons with broad host ranges. Popular replicons from these groups include: RK2 (IncP)...vectors for gene expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https:/...endonucleases on the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https...

...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in... variable and helps ensure the phenotype isn’t singular or associated with only one aliquot/preparation...

...CRISPR nucleases are remarkably precise molecular tools for cutting DNA. But “remarkably precise” does...based on a deeper understanding of the underlying molecular mechanisms involved, without adding any unnecessary...scaled to how much specificity you need for your particular experiment. Step 1: Choose your target well If...

...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103...Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols...

... matter how many times I say this, candidates regularly try to convince me that their search committee...whiskies, but I remember the days of modest postdoc salaries all too well. If there's a group to make money...search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

..., but I always seek out information about the particular audience and venue before tailoring each presentation...peer mentors to check your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities...informal poll by asking for a show of hands on a particular question. I like to ask about how many people...

...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use...

...appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but with some extra...selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk you through the...if the localization and intensity patterns are similar between the two experiments. If not, it might be...

...Mentorship Program work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as... Medical School, where she studies age-related macular degeneration. She is also a blogger who writes ...

...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

...Photocleaveable PhoCl protein for controlling molecular processes with light. Listen to Our Segement on...Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers.  Listen to the Segement on the...

...been an important animal model for research, particularly genetics research, for decades. Its short life...bright burst of light, visible to the naked eye, at regular intervals. This innovation system can help fly ...

... Addgene.org Find basic molecular biology protocols Check out our molecular biology reference pages ...

...the Molecular Cytology group from the University of Amsterdam. Happy Holidays from the Molecular Cytology...

...uses plasmids containing genes from rabies and vesicular stomatitis virus, you may have noticed it’s been...temporary and now on a more lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing...

...two blog posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits ...

...structures demonstrating “the structure is strikingly similar to the design model at atomic resolution” (Kuhlman..., B. L., & Baker, D. (2003). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy. Science...

...be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene... (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038...

... from our free distribution services for their popular constructs, why not let us know? Fill out our referral...if you would like to get the word out about a particular microorganism, microbial technique, or microbial...