We narrowed to 793 results for: lat
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TypeBlog PostPublishedJuly 20, 2017, 2:30 p.m....function, and C. elegans overexpression plasmids to upregulate their expression in specific cells. With a more...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...
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New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....citing plasmids from Addgene help others find them later if they need to use them in their own work, it also...should make it easier for them to find your work later. We also hope that this feature will decrease redundancy... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously established... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....is pictured here…she started from a blank white slate and developed this over 30 minutes, which was fun...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....game for anyone looking to quickly and easily manipulate specific genes. But what if you want to study...said they were able to produce a very diverse population of cells within a lab dish in a matter of a few... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m.... left four years later with a master’s. At the time my degree felt like a consolation prize, but in time... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we ...size of the plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m.... to discover your insights on a topic. Beyond collating other’s data, you can even include your own data...patients was published, Zika researcher Christa Osuna collated Zika case studies, making a meta analysis available... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m.... part of the job, but Caitlin doesn’t work in isolation. “A lot of what we do is work with students who...theses, and occasionally posting something science-related on Facebook. “I think this helped me say with confidence... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog PostPublishedJune 4, 2020, 1:22 p.m....inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the... -
Fostering communication in remote collaborations
TypeBlog PostPublishedApril 21, 2020, 1:14 p.m....person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....identified proteins and enzymes. These compounds will later get tested for their potential agricultural and ...agriculture. For this project the expression of isolated bacterial proteins can be achieved using a bacterial... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m.... data, and I was given a fantastic spreadsheet template to build out a report on. I was able to understand...cultivates trust between coworkers and that trust then translated into this incredible experience. Of course, ... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ... -
Cellosaurus: A Cell Line Information Database
TypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m....LitSuggest Tool Following the “rabbit hole” of related cell lines when annotating a cell line Community...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...