We narrowed to 629 results for: Dos;

...to tell a story - convince your audience you are doing amazing things."  As Sample6 raised more money, ...seeking funding, ask yourself if you really want to do this.” Biotech startups are only successful if they...

... the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are eukaryotes... Plasmids for use in S. pombe, on the other hand, do not require a well defined ORI. Instead, the size...

...’ll need to align all these images once taken. To do so, you will need to include a marker like the Hoechst...needs, your sample, and targets, you might decide to do simultaneous IF instead (Figure 1B). To label all...important consideration when deciding whether you can do simultaneous or sequential labeling is what conjugated...

...exciting trends in your experimental groups, but how do you determine if they deserve that oh-so coveted ...can be run on these tests. All sample comparisons Do you really need to know every pairwise comparison...mathematician to select the right statistical test, just do your homework and happy testing!   References Wissing...

... for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes, plural...reference genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive...you from understanding how the analyses work - you do need to make sure the assumptions in your dataset...end of your analysis process. You’ll still need to do statistical analysis to determine if there are any...

...sequence starts with a 'G', you naturally have one and do not need to add an additional 'G'. Q4: Where are ...) template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ...DMSO or without 5% DMSO. My genomic PCR didn’t work, do you have any suggestions? A14: The Zhang lab recommends...you used in the Science paper (Cong et al., 2013) do you have any suggestions? A15: To date, I have never...

...many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability to...

...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...sure to think about what pieces of the antibody you do and don’t need, and how the protein will interact...

...during your western and your results look… funky? Do you need to start a whole new blot? Never fear: membrane...directly to buffer without a re-wetting step. Important: do not dry your membrane with antibodies on it if you...

...inducer. Since induction via estradiol and progesterone do not affect each other, these systems can be used ...random variation, or noise, in reporter expression. To do so, one places reporter expression under the control... et al. Cell. 2017. PubMed PMID: 29153837. Endosomal pH sensors for STED nanoscopy Article contributed...contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first Addgene plasmid deposit... biosensors for super-resolution microscopy.” Endosomes are historically difficult to study in living ...met some of the criteria necessary for studying endosomes, the Hell lab designed their super-resolution ... meet all of them. These probes had to target endosomes, be exceptionally bright (which is necessary for...