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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 320 results for: POR C

Showing: 301 - 310 of 320 results

  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development of constitutive...methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus, Neisseria spp., Paracoccous...Paracoccus denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp...Xanthomonas campestris   In addition to incorporating broad host range replicons, recombinant vectors...can be expressed. For example, scientists can incorporate interchangeable antibiotic resistance cassettes...

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Barlow-Anacker, A., Bochkov, Y., Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16...encountering flow data in the literature. Data reported from flow cytometry experiments can be a little...correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients. World...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... internal tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events..., D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions...FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in... a cell and depends on the efficiency of BV incorporation by an apoprotein and protein expression level...higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general...

  5. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...1975): 503-517. PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test...although they are reported to have high error rates (10). It is projected that nanopore-based sequencing... similar experimental questions (see below). Importantly, although their role has changed, restriction...lengths because extension is terminated upon the incorporation of a ddNTP. Researchers perform four different...activity as fluorescently labeled nucleotides are incorporated, a method called pyrosequencing. The drive for...sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads the nucleotides...nucleotides of a DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the...

  6. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... easy for a researcher to quickly generate N- and C-terminal epitope tag fusions to a protein of interest...found in your gene of interest) and prevent incorporation of extensive sequences used for cloning into... your research to a variety of experimental opportunities. The plasmids in this kit have been used to ...library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted...are just the tool you need. The plasmids in this portion of the BIOFAB Plasmid Set contain terminators of...strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013. These terminators...Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation...

  7. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...a variety of imaging conditions (155 μs and <0.1 °C in optimal conditions). While B-gTEMP itself is a ...of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence localizes to the plasma...And orthogonal sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size (~1500...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...

  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...in fewer cells being considered for the analysis. (C) A more liberal gate allows more cells to be considered...signals. For this, you’ll need to gate based on the proportion between H and A (or W and A) within the same ... just as fine (Figure 4). As H and A scale in proportion (while H and W do not; see Figure 2), single ...                                                                                                         B.    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can ...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn C., et al. "Recombineering...merely co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...3):937-948. doi: 10.1016/j.ymthe.2020.11.009  Guo C, Ma X, Gao F, Guo Y. Off-target effects in CRISPR/...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...
Showing: 301 - 310 of 320 results