We narrowed to 396 results for: organization Addgene
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TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....directly from A. Victoria and expressed in any organism while still maintaining fluorescence. The protein...where the gene is expressed. Click here to view Addgene’s collection of empty backbones for constructing... such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics...OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs ...microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim...
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Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....From the beginning, one of Addgene’s goals was to ensure the scientific community obtained high-quality...the years to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions...will describe the importance of bioinformatics at Addgene and how our search for solutions led us to a partnership...bioinformatics needs Fig. 1: QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow...than half of these sequences were generated by Addgene as part of our QC process. Automation and continuous...pipelining In many use-cases, including ours at Addgene, the overall purpose of a data pipeline is to perform... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m.... of your gene’s functional biology in a living organism. This is the first post in a three-part series...own mutant flies. Find Drosophila Resources at Addgene The birds and the bees when it comes to fruit ...basic knowledge to start working with flies. Like Addgene, the Drosophila community is highly sharing-oriented... a “pound symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....their presence. They are genetically modified organisms, and GMOs have a bad reputation. The public is...Russell Terriers. More SynBio Reading Read more Addgene blog posts about synthetic biology ... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....of tube for each plasmid we have at Addgene. Each sample at Addgene has four layers of location which can...pages! Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the... a microcentrifuge tube. Once a sample enters Addgene’s lab, a series of events is triggered. Each step...fluctuates throughout the year (see graph below). Addgene scientists inspect each sample and make sure they...takes over and prepares the sample for entry into Addgene’s repository. For long-term storage in our facilities...location There are many different types of samples at Addgene - DNA sent from depositors, bacterial glycerol ... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m.... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences. Gordon ...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease...Glazebrook, 2002). Find plant plasmid resources at Addgene Choosing an expression system Choosing an appropriate...21 September 2018). Additional resources on the Addgene blog Engineering the plant genome using CRISPR...resource page Deposit arabidopsis plasmids with Addgene ...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-Cas system they block can...10.1126/sciadv.1701620 Additional Resources on the Addgene Blog First time CRISPR user? Check out this post...Optogenetic Control of CRISPR Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...Pubmed PMID 17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS...Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for Rabies... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....been an invaluable resource for studying the organization and function of cellular systems. FPs have been...PMCID: PMC4889673. Additional Resources on the Addgene Blog Learn about Fluorescent Protein Timers Check...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular...Use Rosella to Monitor Autophagy Resources on Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent...