We narrowed to 841 results for: abo

...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...part of the answer to many biological questions about the brain. To address this, molecular tools to map...

...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology... Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids ...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...protein activity while also capturing information about spatial and temporal organization. The design use...

...Addgene Blog Learn about techniques for visualizing protein turnover Learn about plasmids for in vivo...

...experiments, therefore, you often must make a choice about what is your top priority: location or efficiency...These Cas proteins have everything we know and love about Cas9, but with broader PAM compatibility. This alternative...

...trials The CRISPR therapies don’t stop with the list above! There are many, many more in the earlier stages... and others we just don’t have the time to talk about. Other treatments currently in clinical trials include...

...cycling. The Pros and Cons Researchers used the tools above to complete a 52-piece assembly of a 40 kb T7 phage... help from DAD. The 35-piece assembly mentioned above had a remarkably high 71% fidelity, while the 52...

...Destination vector (dropdown options depending on above selections) Module set (the set of components you...vectors) Target type (drop down options depending on above selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other...

...blog Find our CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts... posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide Learn more...

...a cell culture model. What we still don't know about Cpf1 In their original paper characterizing Cpf1...Addgene Blog Catch Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac...

...additional helper sequences (2-3). As alluded to above, termination and polyadenylation of Polymerase II...how the Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids ...

... the histone protein of interest (in the figure above, H3 at K9 or K27). Joined to this by a flexible ... in Your Experiments Post Read Other Blog Posts about Fluorescent Proteins  ...

...controls must be run in triplicate.  The nice thing about qPCR is that since it measures relative quantification...number, albeit within a range of RQ values as noted above.   In both applications, results can be affected...

...capacity. For instance, with a packaging capacity of about 6 kb, the 1st generation of AdVs have already a ... CRISPR/Cas9 research! To find more information about the adenoviral delivery of CRISPRS/Cas9 using the...

...Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check out ...

...Check out our blog post on AAV serotypes for more about the tissue tropism of these and other AAVs. ...β-secretase; AF = AMPA.FingR; FingR = fibronectin intrabody generated with mRNA display. Modified from Rivera...

...Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A.,...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

...Routes of administration. In H. Hedrich (Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, ...M. A. (2011). Administration of substances to laboratory animals: routes of administration and factors...

...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

...Addgene blog Learn about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko...