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Showing: 601 - 620 of 801 results

  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers two...single-gene knockdown experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model ...

  3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment! ...Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https:/...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...waste should be autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana...expression disregulation on HeLa cells." Journal of applied phycology27.6 (2015): 2321-2332. 3. Contamination...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... just trying to create a knockout, you might be happy targeting anywhere in an exon. But things get more...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach ...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...

  8. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...feel free to contact us at [email protected]. Happy amplifying! More resources on the Addgene blog ...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...shadows along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to...

  10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ... Sometimes it’s a good way to find out what’s happening in the office next to you. There is an active ...sometimes stupid or uncomfortable things that have happened to them. To have that conversation out in the ...

  11. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

  12. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

  13. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

  14. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and...budget gets a bit thin, small-molecule labeling approaches can provide a fresh infusion of photons for your...

  15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ... tube contains information about that tube’s appropriate location. The barcode also identifies the contents... sample tubes that we use match those of the appropriate deposit. Using barcoded tubes is part of Addgene...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... have created a yeast-based directed evolution approach with some notable improvements. Directed evolution...specific guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and...

  19. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...particles can be identified after negative staining and appear darker than full vector particles.     ...replaced with a new vial of early passage cells. Approximately two weeks post-thaw, cell culture supernatant...

  20. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...easiest to train individuals who are polite, appreciative, and clear about their needs. Remember, your...trainees one day when you are the senior personnel! Happy training! Resources Resources on the Addgene blog...
Showing: 601 - 620 of 801 results