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We narrowed to 847 results for: CAL;

Showing: 601 - 620 of 847 results

  1. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ..., a powerful open-source search engine that can scale to search millions of web resources and handle millions..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms...PI labs and institutions. There are many more technical details that make our new search engine unique...

  2. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification...Well, even though Dam methylation sites are not specifically associated with any restriction modification... These specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient...

  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ... rate would need to be much, much lower before clinical applications could be considered. It is important... efficiency and applicability. Given moral and ethical concerns surrounding such research, it is crucial... experiments are needed before we can consider clinical applications for germline editing. Although this...

  4. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...reprograming influence tumors development? Being pathological active regions, microenvironments shape a tumor...interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6. ...expression predicts early tumor recurrence and poor clinical outcome in human breast cancers." The American...

  5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...developed cloning vectors to harness it. These vectors, called pKIL18 and pKIL19, contained the ccdB gene in frame...ccdB and therefore would die. This procedure dramatically reduces the number of clones that do not contain...and ccdB Survival strains have been developed specifically with ccdB-containing vectors in mind, any plasmid...

  6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ... year, narrow by article type (review, patent, clinical study, research article etc). There are also numerous...conclusions. In any case, the reader should give a critical eye to all results in the relation to how they... biochemistry instructor in the Department of Biological Sciences at Louisiana State University. She is...

  7. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...individually. Enter the BIOROBOT! This robot is called the Qiagen BIOROBOT Universal Platform and it suits... for a mini prep, except it does it on a larger scale. Using the BIOROBOT Universal Platform is ideal ...

  8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ... to be re-engineered for each gene, making wide-scale gene activation seem next to impossible. The development...methods greatly improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum et al....JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional activation by an engineered CRISPR-Cas9...

  9. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...Addgene’s Lab Manager, was taking classes at her local community college when she took her first job at... and hoping to pursue. The choice to not go to medical school was devastating. At the same time, she has...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...., 2021). Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab....genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation), from Addgene to better understand...bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection. ...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...unintelligible to a lay audience. My lay paragraph typically includes a couple of sentences of introduction... rejected on some occasions. Strategize, choose calls that suit your career stage / research area, and...peers and collaborators can also help you get some critical insight into what you're doing wrong. All this...

  12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...remarkable progress on establishing a system for the chemical replication of RNA in the absence of evolved enzymatic... about using CRISPR/cas to address the growing clinical problem of antibiotic resistant bacteria. See ... and Sun Yat-sen University presented two new technical developments. Using the Tet-on system, his lab...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...edits or knock-ins. Editing efficiency decreases drastically once your cut site is more than 10 bp away from... mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a... a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit...

  14. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...sequencing primers for quality control. At such a scale, automation is crucial, both for speeding things...to the same principles listed here. It employs biological best practices. For example, it ranks guides ...published on how to do it best. Scientists are typically advised to mutate the PAM to which the guide binds...

  15. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...but close together in terms of where they are physically located. DNA is then sheared into pieces small...on-target enrichment. 2. Determining the spatial and hierarchical organization of chromatin interaction networks...CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction networks. Think of these...

  16. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...bicistronic vectors take advantage of an element called an Internal Ribosome Entry Site (IRES) to allow...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...journal.pone.0018556. Epub 2011 Apr 29.) PubMed. 5. Scalable signaling mediated by T cell antigen receptor-...

  17. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...-55 °C for the homologous region (calculated using Oligo Calculator). Insertions - To insert short sequences...parental DNA (37 °C for 15 mins). This enzyme specifically cuts methylated DNA, so it will selectively ...

  18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...Liang et al. 2017).  PEG-mediated methods, while technically less difficult to perform, often show low efficiency...ribonucleoprotein electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215...Cell-Type-Specific Gene Editing." Journal of the American Chemical Society 140.21 (2018): 6596-6603. PubMed PMID:...

  19. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...not the case for knock-ins. Knock-in mutations typically require an exact DNA sequence to be incorporated...know that HR is often mutated in cancer cells, specifically in breast and ovarian lines commonly used in...common editing pathway for DNA repair. To tilt the scales in your favor for an HDR edit, you can try one ...

  20. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...culprit for these unusual cases was either 1) a physiological effect of SARS-CoV-2 toward destabilizing one...Adenovirus (and/or to its partner, AAV) or 2) a sociological effect of the COVID-19 lockdowns causing a lack...Adenoviruses: General Features. In Reference Module in Biomedical Sciences. Elsevier. https://doi.org/10.1016/B978...
Showing: 601 - 620 of 847 results