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  1. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...extreme changes in osmotic pressure and pH can destabilize the viral envelope. One drawback of the multiple...

  3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... live cells in real time. Previously, we have described a number of practical considerations that should... herpesvirus.       References 1. Snapp, Erik. "Design and use of fluorescent fusion proteins in cell ...

  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...one temperature. Two Cas12a-crRNA complexes are designed, each binding to one strand of the reverse-transcribed...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...cryo-EM samples from damage at the AWI. Plus, tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...antibody-based technique, validation and proper experimental design are essential. In this article, we’ll go through...steps include: counterstaining, mounting sample on slides, and confocal   Figure 2: High-level overview...

  7. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... – including undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name...impossible deadlines, unnecessary disruptions Destabilization – including failure to acknowledge good work...

  8. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...single enzyme if your insert is flanked on both sides by that enzyme’s restriction sites, but the insert...the orientation of your insert. You may want to design a diagnostic digest for this purpose. Ideally, ...

  9. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... are more involved in the science, experimental design, and execution. Can lead to an independent project...advice Give yourself permission to pursue your own desired path. Careers in science are also not limited to...

  10. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... recognition are a few methods by which tumors sidestep immune surveillance: Suppressing Function Antigen...target is never the answer to the multi-level barricades put up by cancer. As mentioned in this article...

  11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably... the optogenetic constructs Expression of your desired optogenetic construct in the brain region and cell...

  13. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...guide and use it for quick reference when you're designing your next virus experiment. Subscribe to Viral...

  14. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    Published
    Jan. 7, 2020, 1:55 p.m.
    ... because the finalists will receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's...

  15. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured over the...

  16. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ... research, particularly genetics research, for decades. Its short life cycle, reasonably close genetic...

  19. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ... McCarty is the editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also...

  20. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...institution. Requesters will need to provide a brief description of the applicable research project when purchasing...
Showing: 601 - 620 of 785 results