We narrowed to 777 results for: INA
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TypeBlog PostPublishedDec. 2, 2019, 2 p.m....did not affect the function or efficiency of the original APEX (Lam et al. 2014). They named this mutant... technique with proximity labelling to validate binary protein-protein interaction as well as identify...
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Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....BioRender.com The first couple of steps are preliminary steps that are not exclusive to IF, but they ...Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....actually two distinct populations of β-cells that originate in different regions of the pancreas and produce...1935914. PubMed Central PMCID: PMC453137. Menezes, Regina, et al. "From the baker to the bedside: yeast models... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m...., Arner, E., Arner, P., Ashoor, H., Åström, G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A....-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D., & Mori, M. A. (2021). DICER: structure, ... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....level you can click on your state in this map. Finally, the march is meant to inspire scientists to be...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m.... The diversity panel - Equity and inclusion The final and possibly the most important panel of the symposium...quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined a ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m.... genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....with early career science policy groups, attend webinars, and learn more about the field. Read the CCST...accelerator for some hands-on guidance. Check out my webinar via the NC Science Policy student group SciPolPack... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...given the stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al.... -
Antibodies 101: Flow Cytometry
TypeBlog PostPublishedJuly 20, 2021, 1:15 p.m....and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...staining is quicker than indirect staining and eliminates potential non-specific staining that may arise... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m.... the annealed cr:tracrRNA is then mixed with recombinant Cas9 protein to form the ribonucleoprotein complex...the promoter region of the non-HDR allele. For N-terminal tagging, specifically searching for error-free... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....The current classification system relies on a combination of sequence similarity, phylogenetic analysis..., & Ke, A. (2024). Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....use, including all information provided in the original reference library, as well as GC%, reverse compliment... that you have specified. Run your screen! Final note I am very grateful to the researchers letting... -
New Coomassie Protocol Video
TypeBlog PostPublishedOct. 31, 2023, 1:15 p.m....latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies." Hot tip! This protocol can easily... -
Antibodies 101 Series on TikTok
TypeBlog PostPublishedMarch 19, 2024, 1:15 p.m....antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia... -
The 12 Days of CRISPR
TypeBlog PostPublishedJan. 3, 2023, 2:15 p.m....prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m....some time out every once in a while – just read Melina Fan’s post on lunch. Why should our new blog be... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....community, we've partnered with distributors in China, Korea, Japan, India, and Brazil. We've also created... -
The Codon Guide to Synthetic Biology
TypeBlog PostPublishedSept. 14, 2023, 1:15 p.m....probably the papers and textbooks. I’ve selected “seminal” studies for various categories, including CRISPR...