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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 771 results for: IRE;

Showing: 601 - 620 of 771 results

  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

  2. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...They enjoy the fact that each new challenge will require knowledge of new topics and that they will switch...analyses skills were applicable to my cases. I can directly use my understanding of the molecular behaviors...

  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...course of infection. These binding events can be impaired by the electrostatic repulsion of the negatively...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...sequences, which often have absolute position requirements. For example, a KDEL ER retrieval sequence only...for subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina...

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of Rho GTPases...Piatkevich, K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...electron microscopy (cryo-EM) protocols typically require high concentration and purity of the target molecule...particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no...

  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...forward scatter (FSC) and side scatter (SSC). To acquire these measurements, the cytometer illuminates the... “lymphocyte + single cell + live” population, acquired from the previous gating pipeline, about 53% of...

  9. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...to support your work. For example, if your work requires specialized equipment, try finding institutes ... institutes were able to provide the resources required for him to meet his goals. Therefore, he turned...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Similarly, placing UAS upstream of a transgene permits directed expression of that gene in cells that also express...systems, allowing for more complex control over desired gene expression. Take a look at all of the options...

  12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...one since the formation of mEosFP’s chromophore requires a temperature below 30°C and this is not ideal...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve cellular...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

  14. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...

  15. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...helped us manage our data. To quote Jason Niehaus, Director of Data Science and head of our Data Team, “Our...

  17. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...win a hot chocolate party that will warm up the entire lab. We’ll choose 4 winning submissions and deliver...

  18. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...describe newly engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives...

  20. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th day of CRISPR, Addgene...
Showing: 601 - 620 of 771 results