We narrowed to 662 results for: MAL

1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ...expression of ion channels, despite having an otherwise normal genomic background, can even lead to the formation...

2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...great gene therapy delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery...

3. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...should enable researchers to make precise edits in mammalian cells and may decrease worries about off target...

4. Plasmids 101: Golden Gate Cloning

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   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...increased number of fragments, or the ligation of very small/very large fragments, these problems can be overcome...

5. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...references and sorting out what they mean is no small task. As a scientist, it is useful to make literature...

6. A Look at Addgene's QC Process

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   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...plasmids in bacteria — has many points at which small errors can be introduced. Eventually, these errors...

7. Design Tips for Prime Editing

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   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ...2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008), 639–647....

8. Plasmids 101: Modular Cloning

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   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered...

9. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...originally written, the main focus was on editing animal models of disease and cultured cells. For example...

10. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...from viruses that can infect people, cells, and animals. Some of these viruses are completely inert, but...

11. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...cover their annual meetings. 8) Training – Get a formal education Some people get into science communication...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... plasmid is intended to be used. Choose from: mammalian expression, bacterial expression, lentiviral, ...

13. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...staff member for the past three years. pgEd is a small team comprised of individuals from diverse backgrounds...

14. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...intervention that rescues the function of even a small fraction of Arg-mutant proteins could improve health...

15. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...their own but can be produced in E1-containing mammalian cell lines such as HEK293 cells. 1st generation...

16. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...of the plasmid corrects any known phenotypic abnormalities due to the deletion (i.e. the plasmid complements...

17. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...the Barrick lab was able to grow this strain in minimal media supplemented with various caffeinated beverages...

18. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...much total sample you need and the volume of the smaller aliquots you’d like to dispense and you’re on your...

19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...between strand 10 and 11 to yield large (1-10) and small (11) fragments. In several cases an FP(1-10) fragment...

20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...is best used for detecting antigens that are too small to be captured between two antibodies in a sandwich...