We narrowed to 794 results for: Mpl

...reporter. Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive...APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more efficient than the BE3 plasmid containing...

...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...(so called “gDNAs”) rather than the RNA guides employed by Cas9 or Cpf1. These short, 24 base DNA guides...

..., in turn, the big picture of the project. For example, how can future regulations impact your work? What...sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities The table below...

... binding affinity to major histocompatibility complexes (MHC class I and II).  There are multiple algorithms...approach The in silico approach cannot replicate the complexity of antigen processing events that take place ...

...from a month to a year. The researcher remains employed by their home institute, meaning the host university...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

...other ways to make your session more engaging, for example, by using short animations and visuals, or asking...be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables that you...

...breaks down the HMO of interest into its component simple sugars (including lactose), and a lac operon controlled...to determine the amount of HMO originally in the sample. The second sensor is a fusion protein consisting...

...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available...

...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter.  ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural...

...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright...

... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...

... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...

...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce...

...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...

...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

...jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently developed a new orange...cell types at once using existing indicators. For example, you might want to use OCaMP to monitor Ca2+ signals...

...chemical reaction takes place. Figure 2: Simplified chemical reaction catalyzed by luciferase, releasing... proteins to be visualized simultaneously. Complementation systems for examining protein-protein interactions...

...an interview setting, this means persuading an employer that your past work has prepared you to contribute...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...