We narrowed to 629 results for: Ncl

1. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal models, tissue...

2. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ...Different rates of translation by different tRNAs, including those that exhibit wobble base-pairing (a tRNA...

3. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of another and...

4. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ... from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons...

5. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

   Type

   Blog Post

   Published

   July 29, 2025, 1:15 p.m.

   ...PEG-mediated transformation into plant cells. Conclusion & Future Directions TnpB represents a new generation...

6. Twenty Years of Sharing: Expanding Accessibility

   Type

   Blog Post

   Published

   Nov. 21, 2024, 10:47 p.m.

   ...systemic changes, like using alt-text, captions, and inclusive design in our digital content. We have implemented...

7. Hot Plasmids - November 2023

   Type

   Blog Post

   Published

   Nov. 14, 2023, 3:35 a.m.

   ...about the whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage...

8. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ...process. When enough copies of ATP-DnaA are bound, including at the low-affinity sites, the proteins work cooperatively...

9. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...host ranges. Popular replicons from these groups include: RK2 (IncP), RSF1010 (IncQ), and pSa (IncW)  (Schmidhauser...

10. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    Published

    Oct. 13, 2016, 2:30 p.m.

    ...burden from getting overwhelming. These mechanisms include efforts like flexible staffing on case teams, where...

11. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...mycoplasma contamination can be subtle and may include reduced saturation density, decreased rates of ...

12. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and...

13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...dominant-negative and/or toxic. A potential solution is to include an in-frame degron, or degradation sequence, upstream...

14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ...sequence in a plasmid and call it a day. You need to include both sides of the complementary RNA duplex and ...

15. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...axis. This effect causes misleading qualitative conclusions, and it’s also problematic for computational ...

16. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...variants deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins...

17. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...attractive strategy for engineering other enzymes. Conclusion We hope this post has illuminated some of the...

18. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ... sequencing functional regions of the plasmid including the insert. This is essential since mutations ...

19. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput screening...

20. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ... genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of ...