We narrowed to 632 results for: Ncl

...Different rates of translation by different tRNAs, including those that exhibit wobble base-pairing (a tRNA...

...Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of another and...

... from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons...

...activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166...

...whether you realize it or not. These skills might include collaborating, managing, and communicating. After...

... use of other tools they use in research. This includes chat apps like Slack, Microsoft Teams, and Google...

...dissenting opinions is to foster a culture of inclusivity. Work with and value people who think differently...

...burden from getting overwhelming. These mechanisms include efforts like flexible staffing on case teams, where...

...mycoplasma contamination can be subtle and may include reduced saturation density, decreased rates of ...

...about the whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage...

...PEG-mediated transformation into plant cells. Conclusion & Future Directions TnpB represents a new generation...

...systemic changes, like using alt-text, captions, and inclusive design in our digital content. We have implemented...

...process. When enough copies of ATP-DnaA are bound, including at the low-affinity sites, the proteins work cooperatively...

...host ranges. Popular replicons from these groups include: RK2 (IncP), RSF1010 (IncQ), and pSa (IncW)  (Schmidhauser...

...dominant-negative and/or toxic. A potential solution is to include an in-frame degron, or degradation sequence, upstream...

...developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and...

... sequencing functional regions of the plasmid including the insert. This is essential since mutations ...

...high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput screening...

...attractive strategy for engineering other enzymes. Conclusion We hope this post has illuminated some of the...

...sequence in a plasmid and call it a day. You need to include both sides of the complementary RNA duplex and ...