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We narrowed to 847 results for: PLE

Showing: 601 - 620 of 847 results

  1. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...Schultz PG. A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia...

  2. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...many different things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate...responsible for the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC ...from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied by migraines...

  3. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists...help the Mentee feel a sense of accountability to complete action items and make progress. To illustrate ...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ... SARS-CoV-2 interacts with human proteins. For example, we used HEK293T cells in our first study, but ...collaborative science, and making it accessible to all people, not just scientists! *If you are requesting plasmids...plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with your order...

  5. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.        A retroviral mouse CRISPR...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter.  ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural...

  6. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...

  7. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...have tried to modify it to improve yields. For example, transfecting the producer cell line with an expression...were always normalized to their genomic titers). Examples include the use of exo-AAV1 for transducing sensory...

  8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...techniques are continually being refined; it is completely plausible that scientists will eventually have...many things we can navel-gaze over from the last couple of weeks, including whether or not scientists can...history of medical research, there have always been people who unethically experiment on humans, and they ...

  9. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N or C terminal...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright...

  10. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available... particular catalog item is available for order please email [email protected] with the vector name and...

  11. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...about FPs than is currently represented at fpvis (complete spectral information, accession numbers, sequences...the goal of encouraging user contributions (using simple web forms) while also maintaining a relatively ...in the early stages of development, and there is plenty of room for improvement and elaboration.  Current...

  12. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3.   Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2.   Improved trafficking and localization... Multiple Authors...

  13. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ... most familiar bioluminescent organism for most people is the firefly (Photinus pyralis) and perhaps not...luciferase activity as a measure of signaling activity. Multiple response elements are cloned into a plasmid upstream... will result in luciferase expression and more complex cell treatments can be designed to further explore...

  14. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...For safety and production reasons, multiple plasmids with many components are used to package lab grown...with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike LTRs, ITRs are...): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: ...

  15. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...researchers new to GCE aren’t able to successfully complete their experiments and many others are dubious ...universal techniques that researchers can easily implement in their own labs. They provide resources, knowledge...bulletin board on their website specifically so people can talk about GCE. In fact, their first protocol...

  16. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...cool because they can transmit information, for example, from one of the 180 regions to another or from...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled... Multiple Authors...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...them an ideal delivery system for human patients. Multiple viral-based gene therapies have already been approved...

  18. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... look at how we create this content, using the example of our Antibody Guide, which was published alongside...can. We even have a Slack channel just for asking people if they can write or review things and another ...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...

  19. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...specific region of your protein of interest. For example, if you are studying a protein that may bind a ...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...higher than that of polyclonal antibodies. For example, it takes longer to develop a hybridoma than it...

  20. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...of both AsLOV2 and the nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion...manipulation of the signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras...levers to pull to control signaling pathways. For example, if the OptoNB could bind and block a receptor ...
Showing: 601 - 620 of 847 results