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We narrowed to 790 results for: Por

Showing: 601 - 620 of 790 results

  1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...been known about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied ...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...protein attaches to cell surface receptors, other important binding events take place during the course of...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...fusion based on your experimental needs. Equally important, before cloning you must consider whether and ...potentially affect function [5]. We and others have reported that FP cysteine residues form inappropriate disulfide...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...added to the growth medium. Induction timing is important, as you typically want to make sure your cells... optimal because of the high cell-density they support; however, minimal media supplemented with M9 salts...

  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ... to therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters...Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org...

  8. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... out of fashion but is still useful and most importantly, effective. In the research world, there is a...plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing ...

  9. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...purification and minimizes sample loss while also supporting imaging, making it easy to identify dozens or...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an antigen of interest...interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction and release...

  11. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...

  13. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...merely co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... areas of microbial research like metabolism. Transport, biofilms, and much more. Because of it’s BSL-...designed for use in Arabidopsis. There are also important tips for plant transformations and the use of ...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...a pressurized buffer called sheath fluid and transported through tubes or capillaries to a laser. As the...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...

  19. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...refer to Cong et al. (2013) for more details. One important note is that this ‘split RNA’ design is not as.... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

  20. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...
Showing: 601 - 620 of 790 results