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We narrowed to 855 results for: REN

Showing: 601 - 620 of 855 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... plasmid libraries containing combinations of different gRNAs in each plasmid. Each gRNA combination can...et al 2016 and Wong et al 2015. Alan Wong is currently an Assistant Professor at the School of Biomedical...systems. He can be contacted at [email protected].   References 1. Cheng, Allen A., Huiming Ding, and Timothy...

  2. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...plasmid) and any other important features that differentiate this plasmid from its predecessor. Important...sequencing provider. Each sequencing service will have different requirements for volume and concentration, and...Resources on the Addgene Website View our Plasmid Reference Pages Get Plasmid Backbone Information from Our...

  3. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...constructs, assemble standard plasmids parts in different combinations, or other new and exciting applications...constructs in a single, high-efficiency step. References: Sequential amplification of cloned DNA as tandem...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...creates strong, well-directed meaning and delivers strength to the sentence. Below are the typical sections...an opportunity to summarize the limitations of current state of the art technologies. Therefore, take ...address some, if not all, of the limitations of current methodologies. This section sets the stage for ...

  5. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...great hack for finding relevant papers even if you aren’t familiar with the topic.  Meta Meta is a literature...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...

  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...compatible with multiple FP(11) fragments, even from a different base FP (for example, EBFP2(1-10) can be used ... useful. Good luck, and happy experimenting! References and Resources Additional resources on the Addgene...inducible genome editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...transactivator, Tat. For more information on the different generations of lentiviral packaging systems please...experiment, it is recommended that you try a few different ratios of transfer, envelope, and packaging plasmids... Addgene’s website for additional resources. References Kumar M, Keller B, Makalou N, Sutton RE. Systematic...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...tools for genetic engineering. There are several different viral vectors to choose from, for example gamma-retrovirus...expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...mammalian cells with a single viral infection. References Maetzig T, et al. Gammaretroviral Vectors: Biology...

  9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ... distinguish real and artifactual postsynaptic currents (PSCs) this work shows that the artifactual PSCs...changes in TagRFP-T fluorescence intensity at different time points, the authors tracked protein activity... FLIM-FRET acceptors here. See Addgene's FRET reference page for many other common FRET vectors. Murakoshi...

  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...for either a circular or linear assembly. If you aren’t constricted to a specific number of fragments, ...base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see Table 1). ... and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst...

  11. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...a blot, you should not compare the strength of bands from different rounds of detection. Finally, stripping...abundant. If the abundance of your targets isn’t too different, consider antibody affinity: stronger binding ...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...publish a new blog post, and live tweet from conferences. Find more tips on using social media as a scientist...communicate science. You can draw live sketches at conferences like @ATJCagan or create agar art with colorful...active with it, new doors started opening up. My current job as a Marie Curie Fellow is a direct consequence...

  13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, these...deletions at the 3’ end of the target sequence. Cpf1’s strength may lie in its low off-target editing rates, determined...Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...white arrowheads). It is necessary to use two different pairs of target sites for this strategy to work...another. In the field of mouse genetics, FLEx is currently being used to study a wide variety of in vivo ...the genOway press release for more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling...

  15. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...workshops, presentations, and lectures. There are currently around 1,000 active makerspaces around the world...learning and communicating with one another.      Currently our main focus is coding the molecular biology...academic labs, we have the capability to study many different mutants because the Caenorhabditis Genetics Center...

  16. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...format for events and sessions in scientific conferences. They can result in lively discussions with both...the moderator is not a panelist and will play a different role so her ability to control the event is what...spontaneity so a moderator should be prepared with different questions than those on the prep calls, but often...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...blogger, Laura Desrochers! Laura Desrochers is currently a Postdoctoral Scientist at AstraZeneca. She has...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...these vectors is controlled by a different promoter resulting in different levels of expression from each...labelled or modified amino acids that are useful in different downstream applications. Cell-free expression ...

  19. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ... the heat. Isolating male and female flies Differentiating male and female flies is essential for setting...their adult phase. It is important to clear out the parent crosses from the container before the progeny begin...thorax to look like stubble (sb). For a better reference of various fly phenotypes, check out the Classical...

  20. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...image provided by Manuel Gonçalves; made with BioRender.   Delivering gRNA and Cas9 using adenoviral...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...Zamyatnin AA Jr (2016) Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow...
Showing: 601 - 620 of 855 results