We narrowed to 842 results for: RING
-
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....plan to continue updating the feature database. Powering the plasmid maps on Addgene.org with SnapGene ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full...
-
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m.... ITR integrity and prevent unwanted mutations? During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring. How do I validate my ITR sequences? Next-generation... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes....Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell Host Microbe... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m.... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....Everyone Workshop included this interactive activity during their session. Image from Reproducibility for Everyone....Viewing a poster on a screen is challenging, requiring you to zoom in to different parts of the poster... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing. During the meeting, take notes on the agenda points. At... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....delegating for success. Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....Add ethidium bromide (EtBr) to your gel before pouring it. EtBr binds to the DNA and allows you to visualize... topics such as how to set up your digests and pouring/running a DNA gel. More Plasmid Eductional Resources... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....to rely on differences in emission wavelength. Filtering the emission wavelength partially separates the... Mary Gearing... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m.... change allowing high affinity binding to SspB, brings together the split botulinum neurotoxin halves ... to diffuse in and out but prohibit water from entering their hollow interiors. The Shapiro lab discovered... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... scientists can freeze hybridoma cells before culturing or they can clone the genes from the hybridoma... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing... and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids ... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....come up with a new solution. Now, you may be wondering how this all fits in with science. Well, I’ll ...a software product manager does, you might be wondering what the day to day looks like. Check out the ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...