We narrowed to 837 results for: RING
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TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...
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Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes....Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell Host Microbe... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m.... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing. During the meeting, take notes on the agenda points. At... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....delegating for success. Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....Add ethidium bromide (EtBr) to your gel before pouring it. EtBr binds to the DNA and allows you to visualize... topics such as how to set up your digests and pouring/running a DNA gel. More Plasmid Eductional Resources... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m.... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me. Each of the three steps need... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... scientists can freeze hybridoma cells before culturing or they can clone the genes from the hybridoma... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing... and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light... Mary Gearing... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m.... change allowing high affinity binding to SspB, brings together the split botulinum neurotoxin halves ... to diffuse in and out but prohibit water from entering their hollow interiors. The Shapiro lab discovered... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....to rely on differences in emission wavelength. Filtering the emission wavelength partially separates the... Mary Gearing... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....come up with a new solution. Now, you may be wondering how this all fits in with science. Well, I’ll ...a software product manager does, you might be wondering what the day to day looks like. Check out the ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m.... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....however, they tend to be unstable and may be lost during budding. Yeast Centromere plasmids (YCp): These...with yeast, due to high rates of spontaneously occurring resistant mutants and the insensitivity of yeast... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...