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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 846 results for: RING;

Showing: 601 - 620 of 846 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...Expressed episomally long-term? Expressed short-term? Answering these questions will help guide you to the right...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...and Man: A History of Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007...

  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has...

  4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA ...bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with the antibiotic...

  6. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org...

  7. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...

  11. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings...

  12. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing.  During the meeting, take notes on the agenda points. At...

  13. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...delegating for success.  Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ...

  15. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working...

  16. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...the body. Because calcium levels change rapidly during neuronal activity, scientists can use GECIs to ...shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater brightness, Less signal...

  17. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light...  Mary Gearing...

  18. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...to rely on differences in emission wavelength. Filtering the emission wavelength partially separates the... Mary Gearing...
Showing: 601 - 620 of 846 results