We narrowed to 849 results for: RING;

...translation of CRISPR technology, but they would also bring benefits to researchers conducting basic or early... Mary Gearing...

...plan to continue updating the feature database. Powering the plasmid maps on Addgene.org with SnapGene ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full...

...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but...

...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...Expressed episomally long-term? Expressed short-term? Answering these questions will help guide you to the right...

...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...and Man: A History of Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007...

... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has...

...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA ...bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with the antibiotic...

...spatially confined experiments as well as those requiring reversibility that you cannot do with AraC,” says...MA, Ernst L, Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of...

...Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes....Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell Host Microbe...

... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org...

...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested...

...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...

...Everyone Workshop included this interactive activity during their session. Image from Reproducibility for Everyone....Viewing a poster on a screen is challenging, requiring you to zoom in to different parts of the poster...

...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings...

...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing.  During the meeting, take notes on the agenda points. At...

...delegating for success.  Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ...

...Add ethidium bromide (EtBr) to your gel before pouring it. EtBr binds to the DNA and allows you to visualize... topics such as how to set up your digests and pouring/running a DNA gel.  More Plasmid Eductional Resources...

...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working...

...to rely on differences in emission wavelength. Filtering the emission wavelength partially separates the... Mary Gearing...

...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light...  Mary Gearing...