We narrowed to 848 results for: STI;
-
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR-based marker excision method for marking distinct cell clones developed by the Michalis Averof lab...
-
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog PostPublishedJuly 7, 2017, 1:32 p.m....- increasing open source sharing and helping us stick to our core mission. Chapter 13: Outro - remember...stem cell research as an NIH-funded Principle Investigator in the Kotton Laboratory and is the founding... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....specificity engineering. “The RNA binding domain is interesting because by changing certain amino acids you can...lose their solubility. Her lab is currently investigating the issue and hopes to have a better understanding... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m....project involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start...Collaboration and teamwork One of the defining characteristics of nonacademic science careers is that a team... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....individual plasmids to 5,000 different research institutions. This has given us a unique window into technology...plasmid distributed in our 1st year, 2004, and it still gets requested! Fire Lab C. Elegans Kit - Our first... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....anchors for genome engineering applications was still very new. Now, Konermann and her colleagues including...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...repository can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m....new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... many more tips and ideas on how to use these fantastic tools in your experiments. Many thanks to Hongkui... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m....the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can ...at a more-typical scale of tissue culture while still offering the potential for true biological insight... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m.... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....CHimeric IMmune Editing system) to enable the constitutive, ubiquitous knockout of single genes in immune...To demonstrate the power of these systems to investigate in vivo mechanisms, we targeted two phosphatases... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....save you the time that would be spent making and testing entirely new gRNA designs. You can now easily find...variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m.... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose... -
Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m...., designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence for translation ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m.... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical... -
Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m....? This final post in the PI Interview Series investigates how these three PIs reward their team and manage...during the child-rearing years, learning “lots of interesting things from children, their classmates, and parents... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....frequently used pages in your notebooks and go wild with sticky notes and labels. Color code your Post-its and ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae ...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...