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Showing: 601 - 620 of 797 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading... Design of a Western Blot Troubleshooting and Optimizing a Western Blot Resources on Addgene.org Addgene's...

  3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort to...correct CRISPR components for your application, it is time to deliver these components to your target cells...

  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...coli are easily propagated and have a generation time of around 20 minutes, they are perfect for molecular... Rat physiology is also easier to study and at times more closely corresponds with human diseases and...

  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...State-of-the-art FPs (listed in Table 1) were optimized for efficient binding of endogenous BV.  When ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...

  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...desired chimeric mice, you would breed them many times, hoping to produce a mouse with the desired edit...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

  9. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...important that the digest go until completion. The time required for complete digestion varies for different...speeds; after running the gel for some duration of time, these differently sized pieces will be in different...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery...

  13. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about...

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A...

  15. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video...

  16. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...

  17. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube...

  18. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...from producing your own vectors in the lab to optimizing AAVs in your experiment.      Our second edition...

  20. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social media ...
Showing: 601 - 620 of 797 results