We narrowed to 868 results for: TOR

...team compares it to the plasmid information or depositor-provided sequences to confirm the correct plasmid...them. DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the... full-length predicted sequences submitted by depositors. A sample will automatically pass through our...(1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences...

...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...bacteria, however, the protein integration host factor (IHF) assists with the process: it bends the DNA...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing (‘handcuffing’) ...

... weird black pellet's DNA yielded a new CRISPR effector protein they named Cas7-11. Testing showed that.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature. 2021 Sep;597(7878):720-725. doi...

...possess resistance genes survive and multiply. Factors contributing to the development of resistance include...www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely take time to determine if ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...due to loading and transfer errors — not to any factors from before the proteins even hit the gel. But ...all, while overloaded proteins may saturate the detector. In between these extremes lies the linear detection...concentration as well as by antibody dilution — both factors we’ve covered in our previous blog posts (1, 2)...

...site of interest can be cloned into an expression vector with Gibson assembly. While this is a comprehensive...is generated by transforming a backbone phagemid vector into an E. coli strain that is deficient in dUTPase... combine pairs of mutations together to test combinatory effects. One-pot saturation mutagenesis The ...2 instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...

... green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent... of fluorescent proteins derived from Aequorea victoria GFP sticks out and can be considered a linker ...Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric G-proteins...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

...Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-BioID combines... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins... in one experiment a scientist can confirm and monitor the interaction of two proteins while also biotin...Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein...

...have previously used histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...TALENs focused primarily on disrupting CCR5, the receptor that HIV-1 uses to enter a cell. ZFNs and CRISPR...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

...reduced by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...ladder runs off the gel. Luckily, this is easily monitored in real time, as the dye front (and many ladders...resources with examples, like BioRad’s Western Blot Doctor page, or robust discussions, like ResearchGate’...longer time Protein degradation Add protease inhibitors to lysate Insufficient denaturing conditions...

...to sort cells based on the expression of a new receptor that is of interest to you. You found a couple...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...understand if protein:protein interactions could be a factor in your analysis. Example: You are planning an ...Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R, Turley H, Pulford K, Banham AH (2015) The European...

...protocols for plasmid cloning and working with viral vectors, but what we lacked were videos guiding completely...completely new members of the lab or those taking introductory lab courses. The Intro to the Lab Bench series... need a refresher on some best practices for introductory science techniques. It’s also great for teachers...

...improve its stability. Additionally, the choice of vector can impact the expression level of an antibody ...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an... cellular pathways by binding to cell surface receptors. These applications are typically used for investigating...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

...fields.  Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online...

...new primary investigator. Coming up with new project ideas, applying for grants, and mentoring. Chapter ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of chaotropic...kit for your lab is dependent on many different factors as we detailed above, so be sure to pick what kit...

... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...

...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... our Viral Production Process Find other Viral Vectors ...

...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...'ve helped her at Novartis. The importance of mentorship in developing career skills. Outro Many thanks...Addgene Find more management resources in our Mentoring for Scientists Series Learn about Turning Your...

...resources: Preserving scientific assets: We hold a repository of valuable research materials that might otherwise...continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production that labs...continue.  Moving Forward Together Throughout history, the scientific community has demonstrated remarkable...