We narrowed to 856 results for: TOR
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TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....improve its stability. Additionally, the choice of vector can impact the expression level of an antibody ...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an... cellular pathways by binding to cell surface receptors. These applications are typically used for investigating...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...
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Trends in CRISPR and SynBio Technologies
TypeBlog PostPublishedFeb. 4, 2015, 3:58 p.m....fields. Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog PostPublishedFeb. 6, 2018, 2 p.m....new primary investigator. Coming up with new project ideas, applying for grants, and mentoring. Chapter ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of chaotropic...kit for your lab is dependent on many different factors as we detailed above, so be sure to pick what kit... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog PostPublishedJan. 10, 2018, 4:13 p.m....2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... our Viral Production Process Find other Viral Vectors ... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog PostPublishedOct. 17, 2024, 1:15 p.m.... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog PostPublishedOct. 5, 2017, 1:13 p.m....Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...'ve helped her at Novartis. The importance of mentorship in developing career skills. Outro Many thanks...Addgene Find more management resources in our Mentoring for Scientists Series Learn about Turning Your... -
Addgene Stands with the Scientific Community
TypeBlog PostPublishedApril 23, 2025, 1:30 p.m....resources: Preserving scientific assets: We hold a repository of valuable research materials that might otherwise...continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production that labs...continue. Moving Forward Together Throughout history, the scientific community has demonstrated remarkable... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....the virus itself that kills you, but the cytokine storm it ultimately sets off. The virus works its way...cells and turn the cell into a veritable virus factory. Their findings were reported in the Journal of...and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog PostPublishedFeb. 27, 2020, 2:15 p.m....'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...nonprofit, Addgene does not own the material in our repository. We distribute it on behalf of depositing institutions...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,... -
Addgene's Website Update
TypeBlog PostPublishedApril 8, 2024, 2:15 p.m....two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look and a fresh new... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ... -
Top 10 Open Science Developments of 2013
TypeBlog PostPublishedDec. 19, 2013, 3:46 p.m...., even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...preference to journal access granted via a public repository or authors’ own web sites. 9) The year 2013... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent cell lines and any mutations that that...advantages will be revealed in the absence of growth factor. The team assigned the mutations to functional ...significantly increased cell viability, and these “activators” may be the direct drivers of tumor formation... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m...., and young Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages...different ways to succeed – as loner or gregarious; dictator or laissez-faire; theory or practice; business-like... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....biodistribution therefore introduce additional confounding factors when attempting to interpret signals from these...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...experiments like restriction digests. We’ll have a tutorial showing all of the features available on our Snapgene... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....cannot produce them. By using yeast integration vectors (see for example this yeast cloning kit) she intends...expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins... develop technologies to protect fresh water repositories in Mexico. At Addgene we're happy to be able... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...proceeds go into running the repository and serving scientists better. That includes keeping fees as low as ...