We narrowed to 642 results for: VARS

1. Plasmids 101: Protein tags

   Type

   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...between column runs. The TEV protease itself, with various mutations used to increase its stability activity...

2. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   Published

   Aug. 14, 2018, 12:42 p.m.

   ...detection: fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009):...

3. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...

4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...karyotypically normal and are able to differentiate into various cell types of all three germ layers [7]. To further...

5. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...Reference References  del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication...

6. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...the Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance...

7. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   Published

   July 25, 2019, 12:03 p.m.

   ...simple and easy technique to implement. Success might vary between experimenters. Vacuum infiltration (Mortensen...

8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   Published

   May 2, 2019, 12:41 p.m.

   ...manipulation. The ReMOT control system (Receptor-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the...

9. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...some of our Addgenies had an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti...

10. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    Published

    Jan. 17, 2019, 1:38 p.m.

    ...document is edited, and it’s possible to link between various documents. A second example is the integration ...

11. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...Addgene have minor differences due to in silico variations and lack of verification. To address this issue...

12. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis...

13. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...packages needed to travel meant the accessibility varied country to country.   Figure 1: Number of countries...

14. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...re-use or borrow existing code and control how the various pieces were connected, as this enabled us to rapidly...

15. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...then used this information to identify a large variety of related bacteria with similar Cas systems. Some...

16. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre...

17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...focus on what makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a:...

18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...for biosensors. Aptamer biosensors are used in a variety of applications, including detection of environmental...

19. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ...

20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ...ended up being very straightforward to engineer various cell lines from different tissues and mammalian...