...2019). TREE is a real-time, fluorescent based identification system for the isolation of base-edited cell...to BFP Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular...Harris RS (2018) A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9...

... from depositing laboratories and associated publications, but our quality control (QC) standards have...such as 96-well microplates, and thanks to our dedicated product and development teams, frequent updates...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...

...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate... and plasmids. To encourage collaboration and education in protein science, IPI hosts events and training...subtype-specific β1 integrin mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038/s41467...

...setting. CRISPR is often used for animal germline modification, to repair or add in disease-causing mutations...that AAV-CRISPR does not cause obvious germline modification, an important finding given the controversy ...Blog Learn How CRISPR Could Be Used to Treat Cataracts and Cystic Fibrosis Read How Homology Directed...

...tests  Statistical tests fall into two general categories: parametric and non-parametric. In this blog ... exist somewhere within your data; it does not indicate which groups the differences exist between. For...that matter. However, this is oftentimes an oversimplification. If your independent variables are affecting...

...immune response to the development of tumors is a complicated and dynamic phenomenon that is interestingly ...effects, damage healthy cells, and often cannot eradicate metastasis or eliminate the probability of a relapse...based on their level of progression, anatomical location, characteristic (solid localized tumors or systemic...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

...antibody collection, as well as access to IPI educational resources developed to support use of their antibody... Addgene also offers a growing collection of educational resources on our website and blog, including ...

...these tools over the years, the amount of CRISPR educational resources hosted on our website and blog have...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....et. al. (2022) A plasmid-based system for locating and containing escaped flies. Nature 4:1-22. https:/...

...With barcode tracking, you will always be able to locate materials quickly and easily. Learn more about ...Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos Page 5 Science Rap...

...easy tech transfer process to our website and educational resources. We've got twenty years of experience... you know you can search for plasmids from a publication by pasting the paper's title into the Addgene...

.... I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the foundations...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

...Twitter and has helped us update many of our educational resources. Listen to hear all about Professor...speeds science. Chapter 8 - The importance of communicating research findings through social media and other...

...Born to a family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best... 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to go into...

... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

...Intro Chapter 1: Who is Crystal Shih? Crystal's educational background and position at Novartis. Chapter ...Chapter 7: How Crystal developed her science communication skills and how they've helped her at Novartis...

...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...

...plasmids! Each chose their favorite plasmid and replicated it. Can you guess each plasmid? Our lab paid...creative hijinks last year. They showed us their dedication to great customer support by dressing up as our...