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We narrowed to 811 results for: cha

Showing: 601 - 620 of 811 results

  2. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...individual cell clones with different expression characteristics from a population of transfected clones. The...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

  3. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...are they different from mine? What equipment purchases do we prioritize in a lab with both electrophysiologists...Collide: Managing Successfully Across Cultures by Richard D. Lewis Additional resources on the Addgene...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...time consuming to produce, so most labs opt to purchase adenoviruses from dedicated adenoviral production...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...

  5. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Martin, B. K., Calderon, D., Lee, C., Choi, J., Chardon, F. M., McDiarmid, T. A., Daza, R. M., Kim, H.,...Vergara, X., van der Weide, R. H., Morris, B., van Schaik, T., Manzo, S. G., Peric-Hupkes, D., van den Berg...

  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since the discovery... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants...

  8. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...cells for long periods of time. This reduces the chance of integration at random locations that could cause..., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J....

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab. Recent findings...candidates using a synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...setting and no manager will ever be perfect, but the chances of getting a good manager do tend to be higher ...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

  12. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...Knockout plasmids Homologous recombination is a mechanism to accurately repair harmful double stranded breaks... breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of DNA...

  13. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...heard of another double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ...of a single microhomology sequence. For more mechanistic details on HDR and NHEJ, please see the linked...

  14. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...The underlying method was developed in 1902 by Richard Zsigmondy and Henry Siedentopf to enhance the microscopic...using cameras with electron multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor...

  15. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...identification of DNA binding proteins, this method can characterize nucleic acid-nucleic acid interactions at a ...2017). This upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...and likely functions as a bacterial DNA repair mechanism. The pathway is ubiquitous, with successful recombination...placing homologous ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62-66. PubMed...

  17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...generation of the RNP complex.  While one option is to purchase the Cas9 protein and a gRNA from a commercial ...performed rapidly and, in many cases, with reduced chance for off-target effects. As the CRISPR field continues...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...occurring animal model, the RPE65−/− dog, was characterized and found to suffer from early and severe visual.... PubMed PMID: 29983335. 9. Day TP1, Byrne LC, Schaffer DV, Flannery JG, 2014. Advances in AAV vector ...
Showing: 601 - 620 of 811 results