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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 805 results for: cha

Showing: 601 - 620 of 805 results

  1. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...individual cell clones with different expression characteristics from a population of transfected clones. The...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants...

  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...cells for long periods of time. This reduces the chance of integration at random locations that could cause..., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J....

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab. Recent findings...candidates using a synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...setting and no manager will ever be perfect, but the chances of getting a good manager do tend to be higher ...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

  7. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...Knockout plasmids Homologous recombination is a mechanism to accurately repair harmful double stranded breaks... breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of DNA...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...heard of another double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ...of a single microhomology sequence. For more mechanistic details on HDR and NHEJ, please see the linked...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...The underlying method was developed in 1902 by Richard Zsigmondy and Henry Siedentopf to enhance the microscopic...using cameras with electron multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor...

  10. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...identification of DNA binding proteins, this method can characterize nucleic acid-nucleic acid interactions at a ...2017). This upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...and likely functions as a bacterial DNA repair mechanism. The pathway is ubiquitous, with successful recombination...placing homologous ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62-66. PubMed...

  12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...generation of the RNP complex.  While one option is to purchase the Cas9 protein and a gRNA from a commercial ...performed rapidly and, in many cases, with reduced chance for off-target effects. As the CRISPR field continues...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...occurring animal model, the RPE65−/− dog, was characterized and found to suffer from early and severe visual.... PubMed PMID: 29983335. 9. Day TP1, Byrne LC, Schaffer DV, Flannery JG, 2014. Advances in AAV vector ...

  16. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...final readout. When preparing a standard curve, change pipette tips and mix thoroughly by inversion or...dilution series, use consistent dilution factors, change pipette tips regularly, and mix samples very well...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...γ-Retrovirus Guide References Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex...

  18. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...Cancer Data Science team at Broad, they were able to change their analysis to use a corrected genome map. Now...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

  20. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...journal club should aim to have many of the same characteristics as a successful meeting: a clear purpose and...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...
Showing: 601 - 620 of 805 results