We narrowed to 872 results for: fer

...)    Science communication, or science writing, refers to the process of communicating science to laypeople...knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers ...field-specific terminology or language. This can refer both to terms that are only used in the field and...

...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...Gel purify your PCR product and incubate it with buffer, Taq and dATPs at 72C for 10-15min.  When mixing...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

...field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors. Perhaps most ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork...ask questions if you don’t understand something. Offer to help out wherever you can and don’t forget to...

...sequences and lineages, FRET efficiencies, additional references, etc...) and a more robust database structure...address).  One may also simply attach important references that serve to further characterize a protein ...Currently, all submissions also require a DOI referencing primary literature, and every effort is made ...

...media, etc.). The free version of Grammarly also offers basic writing suggestions, like alternative word...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...

...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...

...     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and the New Research...sometimes we benefit and sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator...follows an intriguing cast of characters from five different families over several millennia as their paths...

...developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase reporters. In...in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein...

...there are two overlapping, valid critiques being offered of He’s work: CRISPR/Cas9 genome editing is not...possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory body...self-police” or whether the media and academic conference response to He created a positive feedback loop...

...both individual and community science projects, we offer classes, workshops, book clubs, seminars, and social...the data to characterize the microbiomes of the different samples. The goal is to not only learn something...something about the microbial diversity in different soil conditions/locations but to engage the local community...

...assembly? In the context of cloning, DNA assembly refers to a method of physically joining multiple fragments...generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, there is no...assemblies and improvements in these methodologies offer streamlined and simplified alternatives to traditional...

...used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red. We froze...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...

...hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input...constructs like plasmids. The plexWell™ workflow also offers a highly efficient NGS-based alternative to Sanger...

...sure to thank everyone for their participation and offer encouragement for the next steps (see “action items...employee’s workload, updates on current projects, and offer feedback. The employee should ask for input on projects...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...

...blog post we will discuss how mammalian plasmids differ from their bacterial counterparts, including how...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working.... This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin...

...that pathways projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

...nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles, the functional...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

...components are still there in real life.  Certainly referring to any DNA within the context of a nucleus as ...chromosomal domains, or how stretches of DNA on different chromosomes interact with each other. Chromatin... Note that histone genes frequently have many different variant names, not all of which can be listed ...

...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...