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We narrowed to 811 results for: lars

Showing: 601 - 620 of 811 results

  1. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... of time, cancers become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke ...drug resistance Wood wanted to find out which particular pathways drive resistance to specific drugs in...

  2. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ... meeting with someone to ask about a job or a particular position at their company, do NOT call this an... is a good time to ask personal questions about salary, opportunities for advancement, challenges and ...

  3. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...in open science this year. Here it goes, in no particular order, my top 10 open science developments, 2013...-respected and high-profile universities. We particularly recommend Eric Lander's intro to biology course...

  4. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs...programs SITN runs and describes some of the more popular topics from the Science by the Pint series. Chapter...

  5. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system is the single-color...studies of cell cycle progression, epigenetics and cellular reactions to external stimuli. It could also have...

  10. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...dimerization of HLA B27, involved in the onset of spondylarthritis, using Bioluminescence Resonance Energy Transfer...the University of California, specialising in Molecular Biology. She has worked in a range of labs - cancer...

  11. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less...

  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  13. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248...

  14. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...essential cofactors for many tools used in modern molecular biology like restriction enzymes and polymerases...posts Resources on Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning...

  16. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments...

  17. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature...

  18. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...protein) with amylose resin. The MBP tag, although large, can actually increase expression and solubility...

  19. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...day at Addgene last summer, through the La Vida Scholars summer program. I was unsure whether or not I ...which was the impression I got from the La Vida Scholars tour. After some time had passed, I reached out...
Showing: 601 - 620 of 811 results