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...   Viral vectors So, what do viral vectors have to do with all of these cool tools? Calcium...

...virus to mice, part of your preparation should be doing your best to be well-rested and alert the day of...procedure. Mice move much more quickly than cell cultures do, and tired researchers are accident-prone researchers...

... cell and animal models. AAVs are non-pathogenic, do not integrate into the host genome, and can be specifically...several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome and therefore ...

...had an industry interview before. What should you do to get ready for this interview? Maybe the above ...the same boat, but there are a few things you can do to make the experience less stressful. First, you...

...different epitopes on the target protein, so they do not compete with each other. This antibody is also...interest that is present. Using a Standard Curve To do that calculation, you’ll need to include a standard...

...inducer. Since induction via estradiol and progesterone do not affect each other, these systems can be used ...random variation, or noise, in reporter expression. To do so, one places reporter expression under the control... et al. Cell. 2017. PubMed PMID: 29153837. Endosomal pH sensors for STED nanoscopy Article contributed...contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first Addgene plasmid deposit... biosensors for super-resolution microscopy.” Endosomes are historically difficult to study in living ...met some of the criteria necessary for studying endosomes, the Hell lab designed their super-resolution ... meet all of them. These probes had to target endosomes, be exceptionally bright (which is necessary for...

...fluorescent proteins or localized fusion proteins and do not require dedicated plasmids or constructs. It ...allow for a well-controlled environment, but they do not mimic the natural situation. Measuring photostability...

...ability to target almost every genomic locus, and to do so in vivo. Catalytically dead Cas9 (dCas9) cannot...CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If used with catalytically...

... all have their benefits and drawbacks. Labs that do not routinely produce virus may want to choose more...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide...

...fragments. When designed correctly, the recognition sites do not appear in the final construct, allowing for precise...these sites are eliminated by digestion/ligation and do not appear in the final construct. The destination...