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We narrowed to 816 results for: LAS

Showing: 311 - 320 of 816 results

  1. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ....12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...the gene of interest (blue) in a plasmid backbone. A separate plasmid contains the transposase gene (red...). The original plasmid is empty following the removal of the transposon; the plasmid is then degraded...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent...for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter, which is...

  2. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    .... pBLADEONLY: This plasmid contains only BLADE and is based on the pTRC99a plasmid. It does not include...AraC and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC...reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest, you would replace mCherry...PBAD promoter. If you already have a pBAD33-based plasmid expressing your gene of interest, all you have ... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...Learn more about inducible promoters Visit the Plasmids topic overview page Resources on Addgene.org ...

  3. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...the cytometer illuminates the passing cell with a laser and detects the light scattered by that cell at ...surface. Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent...experienced colleagues.   Figure 1: The laser pulse illuminates the cell and is scattered forward...the time that the cell spent passing through the laser beam. A is simply the area under the resulting curve... Figure 2: As the cell passes through the laser beam and causes it to scatter (FSC/SSC), the detected...), the time the cell needed to pass through the laser (W) and the area under the curve (A).   While... FSC/SSC plot, largely depends on cell type and laser voltage. The voltage describes an electric potential...

  4. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Addgene.org Find Fluorescent Protein Plasmids Check Out Other Hot Plasmid Technologies ...proteins and is driven by an inducible promoter (Plasmid 64542 or 64543) Before translation, both GFP and...contains a CAAX sequence to bind the mRNA to the plasma membrane; this sequence prevents diffusion of the...Since the SunTag-based system tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs... physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation...19213877 PMCID: PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation...1367-71. PMID: 25792328 PMCID: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, ...

  5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...different plasmids. Harvard’s DRSC/TRiP Functional Genomics Resource Center has various plasmids for overexpression...gene of interest is cloned into a plasmid, you can send your plasmid to a company. For a few hundred dollars...Basically, you place your gene of interest in a plasmid and get the vector incorporated into the germline...filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic... dollars, they will inject the plasmid into fly embryos and pick out flies where your transgene has been...incorporated into the germline. Outsourcing the plasmid injections is much easier than doing it yourself...Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they could use their TALEN...particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you...the genome in rapid fashion. The two remaining plasmids in the kit have the rest of the protein in place...fluorescent proteins used to sort the cells after the plasmids have been introduced by transfection or electroporation...protein that binds those 15 base pairs is take four plasmids out of the library in the appropriate combination... ligation with the four inserts and the master plasmid in one step. The kit also has other unique aspects...you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically...

  7. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...1). Plasmids: We generated a series of plasmids that are available from Addgene. These plasmids enable...Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for protein tagging Find validated gRNAs...Detailed protocols describing the use of these plasmids are available here. The Methods section of Nabet...cloning procedures used to construct the deposited plasmids. Molecules: We synthesized dTAG molecules by conjugating...terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality...Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in human cells Resources...

  8. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...problem of sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors, and making...other tissues. At the Nuclear Receptor Signaling Atlas (NURSA), one of our primary goals is to promote ...progress that has been made in the NR field over the last thirty years or so would not have been possible ...development of a vast number expression and reporter plasmids and the free exchange of these vectors between...increased the burden associated with identifying plasmids in the research literature and acquiring them ... feature direct links to listings of available plasmids for our curated NRs and coregulators. We look ...edits the website of the Nuclear Receptor Signaling Atlas (NURSA) at www.nursa.org. Bert O’Malley, M.D.,...

  9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...analyzing the plasmid and viral DNA sequences and expression profile at multiple steps from plasmid amplification...to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While this strategy greatly...recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019). This means that...of this finding, we now include guidance on the plasmid page of DIO/FLEx AAV vectors where this occurs ... mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous pairs of ...specific” promoter, such as the truncated tyrosine hydroxylase (TH) promoter for catecholamine neurons or glial.../FLEx vectors for off-target expression.  Some classes of neurons transiently express TH during development...

  10. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...Allen Institute for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent..., most secreted proteins are produced in the endoplasmid reticulum, modified in the golgi and then transported... on the gene names in the figure below to find plasmids containing that particular gene. ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers Institute...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest...interest in the Allen Institute for Cell Science Plasmid Collection. You can also use the library of full-length...Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM. 2000. The Cell: A Molecular...
Showing: 311 - 320 of 816 results