We narrowed to 841 results for: abo

...First, you’ll need to collect specific information about your library: the size of your library (How many...with Addgene. If you have any specific questions about designing your library amplification protocol, feel...

...Inserts, either precloned or in amplicon form (shown above) are flanked by BsaI sites in an orientation that...Check out NEB's work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org...

... cancer are yielding promising results in the laboratory, and Addgene is accelerating this research by...within that pathway that are of interest to their laboratory. A single mouse click on a gene of interest will...

...cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield ...Topic Page Learn How to Verify Your Plasmid Learn about Common Lab E. coli Strains Additional Resources...

...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

...or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they can be useful...Important considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals...

...Resources on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids...

... FP fragments and, preferably, some information about the structures of the tested PIPs. Many split FPs...neurodegeneration. He now spends his time learning and writing about science whenever he's not off the grid.   References...

...in his laboratory. Learn more here. Additional resources on the Addgene blog Learn more about plasmid...

...specific genomic region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific...additional research conducted in the Hodaka Fujii Laboratory, please also see our interview with Dr. Fujii...

...and detergents.  Organic Solvents  As mentioned above, organic solvents can be used to simultaneously ...permeabilizing with saponin. However, if you are not worried about washing away your target (for example, some cytoskeletal...

... work. After all, a stick-figure internet comic about math probably didn't sound like a great idea to ...  Selecting the method Once you’ve answered the above questions, you can create your short list of options...

...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

... the frontiers of biological imaging.  *Worried about your budget? Email [email protected] Resources on the Addgene Blog Learn About Other Protein Tags Catch Up on Your Fluorescent ...

... Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...

... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic...

...potential applications of Cas13a given what we know about its structure and function? For starters, Cas13a... Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 ...

... expression systems. Keep reading to learn more about the Gateway cloning method and its advantages. An... on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR...

...for base-editing activity The reporters mentioned above all rely on GFP fluorescence and cell sorting using... Additional resources on the Addgene blog Read about other CRISPR reporters Browse our CRISPR blog posts...

...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...