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We narrowed to 814 results for: App;

Showing: 621 - 640 of 814 results

  1. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

  2. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

  3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and...budget gets a bit thin, small-molecule labeling approaches can provide a fresh infusion of photons for your...

  4. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ... tube contains information about that tube’s appropriate location. The barcode also identifies the contents... sample tubes that we use match those of the appropriate deposit. Using barcoded tubes is part of Addgene...

  5. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... have created a yeast-based directed evolution approach with some notable improvements. Directed evolution...specific guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and...

  6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...Motif (PAM), the sequence NGG.  If these conditions apply to your experimental target, SpCas9 and its enhanced...specificity over a single SpCas9. The drawback of this approach is that two sgRNAs targeting opposite strands ...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits...combining advantages of FPs and epitope tags, smFPs appear to be very effective probes for finely visualising...

  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...similar approach would be good for your work. Hopefully this blog helps you with that…and happy flowing...

  11. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...particles can be identified after negative staining and appear darker than full vector particles.     ...replaced with a new vial of early passage cells. Approximately two weeks post-thaw, cell culture supernatant...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...easiest to train individuals who are polite, appreciative, and clear about their needs. Remember, your...trainees one day when you are the senior personnel! Happy training! Resources Resources on the Addgene blog...

  13. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...bioinformatic analysis. At Addgene, we use a mix-and-match approach for sequencing methods and choose the best method...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365. https...

  14. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...workflow - analyzing the data - is often under-appreciated or over-looked.  Synthego has developed a new...analysis Once the analysis is complete, a new screen appears with a graphical representation of the results ...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...papers and then feel ready to write. What actually happened was that each paper taught me a few things and...to read every single paper. I also learned to appreciate the discussion sections of the primary literature...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway...elements to express the gene of interest in the appropriate system Recombines with the entry clone to generate...

  18. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...from random mutations in the genetic sequence that happen to be beneficial, and persist as a result. Why ...are susceptible to the drug, while the few that happen to possess resistance genes survive and multiply...

  19. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...suffer from an inability to easily deliver the appropriate isoform(s) of a given gene, as, many times, the...engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https://doi.org/10.1038...

  20. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...RNAi or CRISPR, non-targeting controls may be appropriate as they allow you to assess the specificity of...us now revisit our experiment. In Figure 1, it appeared as though the shRNA did not knock down expression...
Showing: 621 - 640 of 814 results