...uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted to an alanine...deaminases edit A-to-G.) In short, the impaired Cas9 localizes the deaminase to where the edit is desired, and...to dCas9.  This technique has been exploited to localize factors such as transcriptional activators and...

...even when larger vector genomes are packaged, the physical size of the genomes is still ~4.7kb. But despite...overlapping strategy has a region of homology, typically ~400-1400 bp long between part A and B of the ...overlapping split vector had an improvement in muscle physiological performance.  2. Trans-splicing Overview: For...

...here). Cas9 is commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus...interest (GOI) into plant cells. A) The system typically consists of a Ti plasmid, into which the GOI is...(2020) Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition ...

...blocking off time for different tasks. I use google calendar for this and try to give myself flexibility by...activities that are needed to accomplish these goals. Practically speaking, this means I write everything in a ...down? It’s more viscerally appealing for me to physically cross things out (it just feels good) and I remember...

...clones from that population. You can additionally calculate the minimum number of clones you would have to...consider using a version of Cas9 that has been specifically engineered for reduced off target editing, such...Resources at Addgene: Read our CRISPRGuide Get practical help from our CRISPR Protocols Find gRNA Design...

...used to mutate the PAM sequence (an NGG sequence critical for Cas9 cleavage), thereby rendering the resulting...unique sites and allows for the introduction of specifically designed mutations (not limited to frame shift...temperature in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID...

...vitro, and what obstacles must be overcome for clinical success. ART and HIV-1 reservoirs HIV-1 infects...these methods represent exciting advances in preclinical HIV-1 research that should be pursued in animal... field, the lessons learned in pursuing one technological application can benefit many others and accelerate...

... incredibly important to the stability of the biological research enterprise going forward, in this post...Addgene), that need people who are data oriented, scientifically minded, and able to communicate complex information...underrepresented groups under their mentorship with the same critical eye they place on others within their labs - as...

...sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of epigenetic ...from Addgene. For most of these constructs, catalytically dead modifiers are also available as controls...Resources on the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: How to Experiment...

...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...promoter in vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org/10.1038... Expression specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https...

...transposition: Class I TEs, also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons...in a genome. But there is a type of mobile DNA, called a transposon, that’s a bit hyperactive and likes...

... a laughter program significantly decreased immunological markers of stress in adolescents. It also put...great use of your time even as that lab bench is calling, let me share my top five new favorite science ...

...understanding of how protein folding works and creating biological tools not found in nature. Dr. Baker’s team was...was not based on any existing protein structures, called Top7. Figure 1: Top7 receives the Nobel ...

...of an “electricity aggregation program” for all local households and businesses. Electricity aggregation... energy expenditure here at Addgene. These are critical systems that cannot be removed or substantially...

...:26 : Kwabena's plans for work in Ghana - Using local biomass "waste" to produce biofuels. 23:27 - 25:...foods. 44:15 - 46:34 : Kwabena's goal to build biological science opportunities in Ghana. 46:35 - end :...

...finishing up their time in the PhD Program in Biological and Biomedical Sciences at Harvard University. Ben and...

...September 13, 2018 This massive scale reagent sharing led us to calculate how much time we have saved researchers...

...wear a mask,↔️social distance, 💓show gratitude,☎️ call up an old friend. Remind them you care!#thanks #...viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea...

...with most reagents sent, and the most recent full calendar year When the website is launched, it will show...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...

...E6 regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new ...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? Browse...