We narrowed to 659 results for: MAL
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TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered...
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Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied many many times in a... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....inhibit the S. aureus Cas9, a Cas protein that is smaller and easier to deliver, but so far has few inhibitors... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m.... networking for the day (or whenever you have a small break), write down some notes on the card so you... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m.... often cycled between 37°C and 16°C to provide optimal reaction temps for both the restriction enzyme ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....consider using Gateway if your insert number is a bit smaller, let’s say 4 or less. The list goes on... In this... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....quite broad, detectors will usually only pick up a small portion of that spectra to use for compensation.... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m.... number (if known), antibiotic resistance, and optimal growth temperature are listed, as well as the name... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....optimized for 37°C, and is therefore most suited for mammalian or bacteria studies, whereas GFPS65T is better... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....district’s science curriculum coordinator with minimal luck, but eventually the 8th grade science teachers... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m.... or improved editing ability in a RESCUE system small enough for viral delivery. Find the RESCUE RNA ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....in science if you’re anything other than a white male. “The Nuts and Bolts” is the title of the chapter... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....The Root and Doench lab selected sgRNAs in this optimal window and ranked them based on their optimized... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....benefits of lentiviral vectors have been observed in small trials for other diseases, including the common ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....Select the model system for your research (e.g. Mammalian) Vector Type: What type of experiment are you ... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....inoculated from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5 mL of this... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m.... 2D barcode can store a lot of information in a small space and is ideal for tracking large collections... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....strategy builds on the steps listed above and formalizes your development. You may consider earning a ... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy and things won’t always...