We narrowed to 794 results for: Mpl

...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce...

...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...

... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...

...an interview setting, this means persuading an employer that your past work has prepared you to contribute...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...

...system work? Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput...right thing to do to put these out there. It’s a simple solution. It would be silly not to share it as ...

...that share a similar direction or problem. For example, a group of people who are all interested in making... such as pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or...

...you work with Give more informed feedback (for example, they may be able to hear you present or see your...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

...modified GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background...additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase the available...

...accessible, anyone could present the material. For example, Addgene’s contributions by Tyler Ford included... and matched to serve a specific audience. For example, I joined this effort later in the year and in ...

...reluctance to share their work too openly too soon or simply a lack of awareness of the benefits of preprints...can! At the time of writing, Mike Lacy was also employed by the American Society for Cell Biology in a ...

...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

...greatly impacting the level of gene expression. For example, the synthesis of deoxychromoviridans requires ...products is needed, such as within a multi-protein complex, stabilized promoters can eliminate variability...

...250 nm), and studying endocytosis is further complicated by the reliance on pH-based biosensors used to...rapid and reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying...

...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...

... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

...being a grad student to an employee, and being treated like an actual employee, was really reaffirming.”...

...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...engineered Cas9 with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by...

...a method that uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a ...a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse transcriptase...

...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal Antibody Sequences...